HISTOLOGY CORE

组织学核心

基本信息

批准号：
7347943
负责人：
DAVID K. RYUGO
金额：
$ 27.68万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-12-01 至 2012-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7347943
关键词：
Behavioral Collaborations Computer software Data Data Collection Development Digital Photography Educational process of instructing Electron Microscope Electron Microscopy Electrons Equilibrium Equipment Faculty Fluorescence Fostering Foundations Frozen Sections Goals Hand Hearing Histologic Histology Image Imagery Individual Instruction Investigation Laboratories Methods Microanatomy Microscope Microtome - medical device Mission Molecular Nature Neuraxis Neurobiology Neurophysiology - biologic function Neurosciences Peripheral Physiological Plastics Postdoctoral Fellow Preparation Procedures Process Research Research Personnel Research Project Grants Research Technics Resource Sharing Resources Science Scientist Sensory Services Smell Perception Staging Staining method Stains Structure Students Techniques Time Tissues Training concept cost digital experience immunocytochemistry implementation research light microscopy meter new technology sample fixation skills tissue preparation tissue processing tool

项目摘要

The goal of this Core is to provide faculty in the Center for Hearing and Balance, and associated laboratories, with access to histological equipment and expertise and assistance. The visualization and quantification of structural features that correlate with physiological, developmental, and/or plastic phenomena within the peripheral and central nervous systems provide fundamental data necessary to infer basic mechanisms of neural function. But with the increasingly specialized nature of science, the expense and difficulty of such studies can place them out of reach for most laboratories. Common resources foster collaborations between scientists with different skill sets and can be the key to implementing exciting research projects that bridge different levels of analysis. Anatomical data frequently support this bridge. The Histology Core will continue to provide assistance as well as access to, and instruction in the tools and resources required for tissue preparation and sectioning for light microscopy. This includes both plasticembedded and frozen sections. The Core provides and maintains a light/fluorescence compound microscope with digital camera for image acquisition and auxiliary workstations for analysis. In addition the Core will enable ultrastructural studies through the services of an experienced electron microsopist.

该核心的目标是为听力和平衡中心以及相关的人员提供教职人员实验室，可以获得组织学设备、专业知识和援助。可视化和与生理、发育和/或塑性相关的结构特征的量化周围和中枢神经系统内的现象提供了推断所需的基本数据神经功能的基本机制。但随着科学的日益专业化，费用此类研究的难度使得大多数实验室无法进行。共同资源培育具有不同技能的科学家之间的合作可能是实现令人兴奋的关键连接不同层次分析的研究项目。解剖学数据经常支持这种桥梁。组织学核心将继续提供帮助以及工具和指导。组织制备和光学显微镜切片所需的资源。这包括塑料嵌入式和冰冻切片。核心提供并维持光/荧光化合物带有用于图像采集的数码相机和用于分析的辅助工作站的显微镜。此外 Core 将通过经验丰富的电子显微镜专家的服务实现超微结构研究。