JEM-123O Electron Microscope

JEM-123O电子显微镜

• 批准号: 6580573
• 负责人: DAVID K. RYUGO
• 金额: $ 42.62万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6580573
• 关键词: biomedical equipment purchase; electron microscopy; neurobiology; structural biology

DESCRIPTION (provided by applicant): We are requesting funds to purchase an electron microscope to replace our current 17-year-old one that has become increasingly unreliable. The proposed microscope will be used by a group of 11 users primarily from the Departments of Otolaryngology-Head & Neck Surgery and Ophthalmology at the Johns Hopkins University School of Medicine. A total of 13 projects will be supported by 8 RO1, 2 KO8, 1 K23, and several nonfederal grants. The purchase of this microscope is crucial for the continued progress of research supported by NIH programs. The breadth of the projects is broad but unified by a foundation in structural neurobiology. Projects include (a) studies of the synaptic organization and plasticity of the mammalian auditory system; (b) histopathologic studies of the human eye; (c) ultrastructural examinations of sensory receptors and associated structures of the eyes and ears in normal, pathologic, and/or genetically altered animals; (d) localization of molecules (cytoskeletal and synaptic proteins, growth factors, or ion channels) in eyes and ears that pertain to normal function; (e) identification of growth factors that enhance photoreceptor growth; and (f) gene transfer experiments involving factors that influence laryngeal nerve growth, reinnervation, and muscle development. Conceptual and technical approaches utilized by the different research groups emphasize the common themes that link these various projects. Our work is highly dependent upon electron microscopy, and the proposed purchase will provide its users with a reliable instrument that is equipped with a number of advanced features that are currently unavailable anywhere at our institution. Award of this instrument will have an immediate and positive effect on the productivity of these projects. It will serve to catalyze interdisciplinary discussions among users, provide a valuable teaching tool for our trainees, and enhance the progress of biomedical research on hearing, vision, and voice.

描述（由申请人提供）： 我们正在申请资金购买一台电子显微镜，以取代我们现有的已经使用了 17 年、已经变得越来越不可靠的电子显微镜。拟议的显微镜将由主要来自约翰霍普金斯大学医学院耳鼻喉头颈外科和眼科的 11 名用户使用。总共 13 个项目将得到 8 个 RO1、2 个 KO8、1 个 K23 和多项非联邦拨款的支持。购买这款显微镜对于 NIH 项目支持的研究的持续进展至关重要。 这些项目的广度很广泛，但以结构神经生物学为基础统一起来。项目包括 (a) 研究哺乳动物听觉系统的突触组织和可塑性； (b) 人眼的组织病理学研究； (c) 正常、病理和/或基因改造动物的感觉受体和眼睛和耳朵的相关结构的超微结构检查； (d) 眼睛和耳朵中与正常功能有关的分子（细胞骨架和突触蛋白、生长因子或离子通道）的定位； (e) 鉴定促进光感受器生长的生长因子； (f)涉及影响喉神经生长、神经支配和肌肉发育的因素的基因转移实验。不同研究小组使用的概念和技术方法强调了连接这些不同项目的共同主题。我们的工作高度依赖电子显微镜，拟议的购买将为用户提供可靠的仪器，该仪器配备了许多目前我们机构任何地方都无法提供的先进功能。该工具的授予将对这些项目的生产力产生直接和积极的影响。它将促进用户之间的跨学科讨论，为我们的学员提供有价值的教学工具，并促进听力、视觉和声音生物医学研究的进展。