TRANSMISSION ELECTRON MICROSCOPE

透射电子显微镜

• 批准号: 2283826
• 负责人: DAVID S WILLIAMS
• 金额: $ 10万
• 依托单位: TRUSTEES OF INDIANA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-16 至 1995-05-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2283826
• 关键词: biomedical equipment purchase; cell biology; molecular biology; neurobiology; transmission electron microscopy

A current model transmission electron microscope (TEM) is requested for use in a central campus multi-user facility. The TEMs presently being used were both purchased in the l96Os, and have become inadequate for our present needs. They do not provide some of the functions required by some of the major users. Repair and maintenance have become severe problems, with disruptive "down-time" a common occurrence. The need for an efficient multi-user instrument has become particularly urgent due to a significant recent increase in the number of researchers in a variety of Departments on campus now employing TEM in their research. The requested model TEM, a JEM 1010, has the required functions that our present TEMs lack -specimen tilt, high contrast, minimum dose system - as well as a number of desired improvements in image quality and manipulation. The user-friendliness of the TEM makes it suitable as an efficient multi-user instrument; it would be housed in a new EM suite in the newly-constructed Institute for Molecular and Cellular Biology building, and thus be available as a central campus facility. Given their unreliability alone, neither of the present TEMs would be suitable for this facility. Centralization of our EM facility will optimize our resources, such as technical personnel, and provide better access to TBM for researchers from Departments that do not have a TEM. The requested grant is for just over half of the cost of the TEM; Indiana University will pay for the balance, as well as for a full service contract and the salary of a full-time EM technician. The user group is made up of ten different research labs in five different Departments. The NIH-funded projects that would use the new TEM involve the study of: (i) Cellular processes in photoreceptors; (ii) Control of microtubule function in development; (iii) Mechanisms and functions of subcellular motility; (iv) Analysis of protein kinase C's role in learning in Hermissenda; (v) Neurogenesis in Drosophila; (vi) Protein kinase C in photoreceptors; (vii) Steroids and trophic factors in an aging neuromuscular system; and (viii) Radiation repair and meiosis in Coprinus. Two projects for which NIH funding is being sought are: (i) Control of asymmetry in cell division during development; and (ii) Ocular toxicity studies involving the corneal epithelium.

需要当前型号的透射电子显微镜 (TEM) 在中心校园多用户设施中使用。目前使用的 TEM 两者都是在l96Os购买的，并且已经不足以满足我们的需要 目前的需要。它们不提供某些功能所需的功能 的主要用户。维修保养成为严重问题， 破坏性的“停机”现象屡见不鲜。需要一个高效的 由于重大问题，多用户仪器变得尤为紧迫 最近各部门的研究人员数量有所增加 现在校园里正在使用 TEM 进行研究。所请求的模型 TEM，a JEM 1010，具有我们现有 TEM 所缺乏的所需功能 - 样本 倾斜、高对比度、最小剂量系统 - 以及一些所需的 图像质量和操作方面的改进。用户友好性 TEM 使其适合作为高效的多用户仪器；它会 安置在新建研究所的新 EM 套件中 分子和细胞生物学建设，因此可作为 中心校园设施。仅考虑到它们的不可靠性，两者都没有 目前的 TEM 适合该设施。 EM 的集中化 设施将优化我们的资源，例如技术人员，以及 为那些没有相关部门的研究人员提供更好的 TBM 访问权限 有一个TEM。所申请的补助金仅相当于该项目成本的一半多一点 透射电镜；印第安纳大学将支付余款以及全额费用 服务合同和全职 EM 技术人员的工资。用户 该小组由五个不同的十个不同的研究实验室组成 部门。 NIH 资助的使用新 TEM 的项目涉及 研究： (i) 光感受器的细胞过程； (ii) 控制 发育中的微管功能； (三) 机制和职能 亚细胞运动； （四）蛋白激酶C在学习中的作用分析 在赫米森达； (v) 果蝇的神经发生； (vi) 蛋白激酶C 光感受器； (vii) 衰老过程中的类固醇和营养因子 神经肌肉系统； (viii) 鬼伞的辐射修复和减数分裂。 NIH 正在寻求资助的两个项目是： (i) 控制 发育过程中细胞分裂的不对称性； (ii) 眼部毒性 涉及角膜上皮的研究。