Kinesin in photoreceptors cells

感光细胞中的驱动蛋白

• 批准号: 7382723
• 负责人: DAVID S WILLIAMS
• 金额: $ 38.5万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2011-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7382723
• 关键词: Cell Culture Techniques; Cell Death; Cell physiology; Cells; Cellular biology; Characteristics; Cilia; Complex; Development; Distal; Genes; Genetic; Genetic Models; Goals; Growth; In Vitro; Kinesin; Kinetics; Knockout Mice; Lead; Life; Lighting; Link; Membrane Proteins; Microtubules; Modeling; Molecular; Motor; Movement; Mus; Mutant Strains Mice; Opsin; Photoreceptors; Physiological; Protein translocation; Proteins; RNA Interference; Retinal Diseases; Role; Suggestion; System; Testing; Transport Process; base; cellular imaging; experience; in vivo; inherited retinal degeneration; insight; kinetosome; protein complex; protein transport; research study; response; skills; tool

DESCRIPTION (provided by applicant): Kinesins are microtubule motor proteins that generate intracellular movement essential for many fundamental cellular processes. The long-term goal of this project is to understand the function of kinesin-2 in the photoreceptor cilium. The current application is based on three new developments: (1) A newly established model of opsin transport in cilia that can be studied by live-cell imaging; (2) Findings that link known retinal disease genes to kinesin-2 and ciliary transport; and (3) Refinement of the genetic model so that loss of kinesin-2 can be studied prior to photoreceptor cell death. We propose to capitalize upon these new findings and developments by: (1) Testing two competing hypotheses for how kinesin-2 generates opsin transport along the cilium, and testing the roles of different proteins in the transport of opsin along the photoreceptor cilium. (2) Determining the contribution of kinesin-2 in different (non-opsin) transport processes related to the photoreceptor cilium. The results of these studies will lead to a better understanding of critical cellular processes in the photoreceptor cilium, and thus provide important new insight into a major group of inherited retinal degenerations.

描述（由申请人提供）：驱动蛋白是微管运动蛋白，可产生许多基本细胞过程所必需的细胞内运动。该项目的长期目标是了解驱动蛋白-2 在光感受器纤毛中的功能。目前的应用基于三个新进展：（1）新建立的纤毛中视蛋白运输模型，可以通过活细胞成像进行研究； (2) 将已知的视网膜疾病基因与驱动蛋白-2 和睫状体运输联系起来的发现； (3) 完善遗传模型，以便可以在感光细胞死亡之前研究驱动蛋白-2 的丢失。我们建议通过以下方式利用这些新发现和进展：（1）测试关于驱动蛋白-2如何沿着纤毛产生视蛋白运输的两个相互竞争的假设，并测试不同蛋白质在沿着光感受器纤毛运输视蛋白中的作用。 (2) 确定驱动蛋白-2 在与光感受器纤毛相关的不同（非视蛋白）运输过程中的贡献。这些研究的结果将有助于更好地理解光感受器纤毛中的关键细胞过程，从而为遗传性视网膜变性的主要群体提供重要的新见解。