CORE B: PROGENITOR CELL ANALYSIS CORE

核心 B：祖细胞分析核心

• 批准号: 7960391
• 负责人: Pradeep Sathyanarayana
• 金额: $ 8.48万
• 依托单位: MAINEHEALTH
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960391
• 关键词: Cell Cycle; Cells; Centers of Research Excellence; Color; Computer Retrieval of Information on Scientific Projects Database; Computer software; Coupled; Flow Cytometry; Funding; Genetic; Grant; Institutes; Institution; Investigation; Maine; Metabolic; National Center for Research Resources; Population; Property; Publications; Regenerative Medicine; Research; Research Personnel; Resources; Services; Side; Source; Staining method; Stains; Stem cells; System; United States National Institutes of Health; Work; base; cell analyzer; embryonic stem cell; progenitor; programs; research and development; stem; stem cell biology; success

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. For this Core, one prime function is to provide high level flow cytometric capabilities to analyze progenitor cell populations and progeny based on immunological, genetic, physical, and/or metabolic properties. These capabilities are of importance in working with rare stem and progenitor populations, but also find excellent application in cell and biomedical investigations at-large. Specifically, this Core is equipped with a Becton Dickinson FACS-Calibur flow cytometer that enables 4-color (immuno)staining plus forward and side scatter analyses. It's also coupled to dedicated workstations with high-end software for population analyses at the single-cell level, including cell cycle parameters. Flo-Jo, Win-List and related programs are also supported to facilitate publication-quality graphics of complete cytograms. With the continuing success of our Center (and Institute) highly productive use of our flow cytometry system has increased markedly (to the extent that we've recently applied for Maine state R & D bond funds to purchase a second BD system). For this aspect of Core B, and to allow ES cell as well as FACS and MACS components to develop in parallel with strong initial support, this latter set of facilities and services (ES cell, FACS, MACS) have been re-framed in year-6 (via NCRR approval) as separate-standing Core E. This also has allowed Core B to begin to develop multi-well high-throughput fluorimetry / luminometry (newly acquired modulus system plus injectors) and to make efficient day-to-day use of a versatile Vi-Cell cell analyzer.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 对于该核心，一个主要功能是提供高水平的流式细胞术功能，以根据免疫学、遗传、物理和/或代谢特性分析祖细胞群和后代。这些功能对于处理稀有干细胞和祖细胞群体非常重要，而且在整个细胞和生物医学研究中也有出色的应用。具体来说，该 Core 配备了 Becton Dickinson FACS-Calibur 流式细胞仪，可进行 4 色（免疫）染色以及前向和侧向散射分析。它还与配备高端软件的专用工作站相结合，用于单细胞水平的群体分析，包括细胞周期参数。还支持 Flo-Jo、Win-List 和相关程序，以促进完整细胞图的出版质量图形。 随着我们中心（和研究所）的持续成功，我们的流式细胞术系统的高效使用显着增加（以至于我们最近申请了缅因州研发债券基金购买第二个 BD 系统）。 对于核心 B 的这一方面，并​​允许 ES 细胞以及 FACS 和 MACS 组件在强大的初始支持下并行开发，后一套设施和服务（ES 细胞、FACS、MACS）已于今年重新构建-6（通过 NCRR 批准）作为独立的 Core E。这也使 Core B 能够开始开发多孔高通量荧光测定/发光测定（新获得的模量系统加上注射器）并提高日常工作效率使用多功能 Vi-Cell 细胞分析仪。