CORE B: STEM & PROGENITOR CELL SEPARATION & ANALYSIS CORE

核心B：STEM

• 批准号: 7720702
• 负责人: Pradeep Sathyanarayana
• 金额: $ 11.76万
• 依托单位: MAINEHEALTH
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-04 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720702
• 关键词: Arts; Biology; Blood Vessels; Cell Maintenance; Cell Separation; Cells; Centers of Research Excellence; Collection; Computer Retrieval of Information on Scientific Projects Database; Critiques; DNA; Funding; Funding Mechanisms; Genetic; Genomics; Grant; Hand; Individual; Institutes; Institution; Instruction; Investigation; Magnetism; Metabolic; Mus; Population; Preparation; Property; Proteins; Reagent; Recruitment Activity; Research; Research Personnel; Resources; Services; Source; Specific qualifier value; Stem Cell Research; Stem cells; Study Section; System; Training; United States National Institutes of Health; Work; base; cell type; embryonic stem cell; human embryonic stem cell line; instrument; quality assurance; stem; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. For this Core, one prime function is to provide state-of-the-art capabilities for the isolation and analysis of progenitor cell populations based on immunological, genetic, physical and/or metabolic properties. These capabilities are of particular importance in working with rare stem and progenitor cell populations, but also find excellent application in cell and biomedical investigations at-large. This Core is now equipped with dedicated instruments for the isolation (or depletion) and analysis of such cell types. This includes a Becton Dickinson FACS-Calibur flow cytometer, a new FACS-Aria cell sorter, and Miltenyi MACS System (Automated Magnetic Cell Sorter) (with each purchased via Institute matching fund mechanisms). Additional users are Institute- wide, including investigators in MMCRI's Vascular Biology COBRE. As emphasized in recent NIH study section critiques, Core B is a critically important and highly productive component of our Stem Cell COBRE Center. It also has been an important tool for our successful recruiting efforts. Recently, Core B has developed a parallel resource for investigations involving the use of embryonic stem (ES) cells. In particular, certain services, technical support, and hands-on training for ES cell research are provided. Specified Collections of mouse ES cells and NIH-approved human ES cell lines are maintained with appropriate quality assurances. Support further includes instruction in ES cell maintenance, transduction, and differentiation. This Core component also provides a service of short-term culture and manipulation of ES cells for individual investigators, plus ready access to ES cell reagents (RNA, genomic DNA, protein preparations).

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 对于此核心，一个主要功能是提供最新的功能，以基于免疫，遗传，物理和/或代谢特性的祖细胞群体分离和分析。 这些能力在与稀有的茎和祖细胞群体一起工作中尤为重要，但在整个细胞和生物医学研究中也很有应用。现在，该核心配备了用于隔离（或耗尽）和分析此类细胞类型的专用仪器。这包括Becton Dickinson Facs-Calibur流式细胞仪，一个新的FACS-ARIA细胞分选仪和Miltenyi MacS系统（自动磁细胞分系程序器）（每次通过研究所匹配的基金机制购买）。 其他用户是研究所，其中包括MMCRI的血管生物学鞋带的研究人员。 正如最近在NIH研究部分的批评中强调的那样，核心B是我们干细胞鞋底中心的重要组成部分。 这也是我们成功招募工作的重要工具。 最近，Core B开发了一种并行资源，用于研究涉及使用胚胎茎（ES）细胞的研究。 特别是，为ES细胞研究提供了某些服务，技术支持和动手培训。 通过适当的质量保证，维持小鼠ES细胞和NIH批准的人ES细胞系的指定集合。 支持进一步包括有关ES细胞维护，转导和分化的指导。 该核心成分还提供了用于单个研究人员的短期培养和ES细胞操纵的服务，以及现成的ES细胞试剂（RNA，基因组DNA，蛋白质制剂）。