PATHOLOGY CORE FACILITY

病理学核心设施

• 批准号: 7959757
• 负责人: WILLIAM JM HRUSHESKY
• 金额: $ 2.96万
• 依托单位: UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959757
• 关键词: Adenomatous Polyps; Anatomy; Area; Biological Models; Biology; Cells; Centers of Research Excellence; Characteristics; Colon Carcinoma; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Diagnostic Neoplasm Staging; Dysplasia; Fostering; Funding; Grant; Grouping; Histology; Histopathologic Grade; Human; Image; Institution; Large Intestine; Learning; Lesion; Malignant - descriptor; Malignant Neoplasms; Mentors; Molecular; Mucous Membrane; Mus; Paraffin Embedding; Pathologic; Pathologist; Pathology; Pathway interactions; Patients; Research; Research Personnel; Resources; Sampling; Slide; Source; Staging; Staining method; Stains; Tissue Stains; Tissues; Tumor Biology; Tumor Tissue; Tumor stage; United States National Institutes of Health; Work; anticancer research; experience; interest; intestinal crypt; neoplastic; sample fixation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Pathology Core provides professional histo-pathological tissue review and pathologist-guided tissue examination and description. This prominently includes staging of tumors appearing in model systems, grading of histopathologic malignant change (dysplasia) within these neoplastic tissues, quantification of the extent of tissue neoplastic or dysplastic change, and the extent of tissue plane invasion by these neoplastic/dysplastic cells. Two core directors, who are cancer biologists with, respectively, two and three decades of tumor biology experience, will work with target PIs, so they will provide adequate quality and numbers of experimental samples with proper control tissues for adequate histo-pathologic review. Tissue blocks and first cut slides provided by target PIs for pathological review from the Histology/Imaging Core Facility will be evaluated by the core director(s) and the Pathologist who will determine whether stained tissues sections are of adequate quality to proceed. If quality of the sections and their staining is adequate, full review will proceed. If the quality of fixation, embedding, sectioning and/or staining is not good enough, the blocks will be obtained, recut and restained until adequate quality is achieved and the best tissue areas are selected. The Pathology Core Facility will communicate with the project PI and mentors to learn about the molecular pathways being examined, in order to choose the best anatomic region most likely to express the most characteristic changes. The Facility will examine, photograph and describe the tissue and cell groupings most likely to express the biology of interest. Cells of intestinal crypts, normal mucosa, and adenomatous polyps may be identified and examined in several distinct areas in each paraffin embedded mouse tissue, and in several anatomic areas of the small and large bowel. The availability of the Pathology Core Facility will assure that all mouse lesions will be professionally examined, staged, graded, and categorized as to their malignant characteristics and potential, just as are colonic tissues and tumors obtained from human patients. The rigorous and consistent pathological interpretation of cancer-related changes in mouse tissues will add strength to all collaborative work fostered by the COBRE group.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 病理学核心提供专业的组织病理学组织审查和病理学家指导的组织检查和描述。这主要包括模型系统中出现的肿瘤的分期、这些肿瘤组织内的组织病理学恶性变化（发育异常）的分级、组织肿瘤或发育异常变化的程度的量化以及这些肿瘤/发育异常细胞侵入组织平面的程度。 两位核心主任是分别拥有二十年和三十年肿瘤生物学经验的癌症生物学家，他们将与目标 PI 合作，因此他们将提供足够质量和数量的实验样本以及适当的对照组织，以进行充分的组织病理学审查。由目标 PI 提供的用于组织学/成像核心设施病理审查的组织块和首次切割载玻片将由核心主任和病理学家进行评估，他们将确定染色组织切片的质量是否足以继续进行。如果切片及其染色的质量足够，将进行全面审查。如果固定、包埋、切片和/或染色的质量不够好，则将获得块，重新切割并重新染色，直到达到足够的质量并选择最佳的组织区域。病理学核心设施将与项目 PI 和导师沟通，了解正在检查的分子途径，以便选择最有可能表达最具特征性变化的最佳解剖区域。该设施将检查、拍摄和描述最有可能表达感兴趣的生物学特征的组织和细胞群。可以在每个石蜡包埋的小鼠组织的几个不同区域以及小肠和大肠的几个解剖区域中识别和检查肠隐窝、正常粘膜和腺瘤性息肉的细胞。 病理学核心设施的可用性将确保所有小鼠病变都将根据其恶性特征和潜力进行专业检查、分期、分级和分类，就像从人类患者获得的结肠组织和肿瘤一样。对小鼠组织中癌症相关变化的严格且一致的病理学解释将为 COBRE 小组开展的所有合作工作增添力量。