ANIMAL CORE

动物核心

基本信息

批准号：
7959825
负责人：
William L. Woolverton
金额：
$ 21.51万
依托单位：
UNIVERSITY OF MISSISSIPPI MED CTR
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-07-01 至 2010-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7959825
关键词：
Address Adult Affect American Animal Experimentation Animals Antidepressive Agents Autopsy Behavioral Behavioral Symptoms Blood specimen Brain Caring Chronic Chronic stress Collection Computer Retrieval of Information on Scientific Projects Database Disease Dissection Doctor of Philosophy Ethics Faculty Fluoxetine Freezing Funding Glutamates Grant High Pressure Liquid Chromatography Human Individual Institution Lead Life Long-Term Effects Medical Medical center Mental disorders Mississippi Moods Neuraxis Neurosciences Pharmaceutical Preparations Process Research Research Personnel Resources Sampling Serotonin Source Supervision System Thinking Training United States National Institutes of Health Universities analytical method brain tissue depression effective therapy experience high standard liquid chromatography mass spectrometry method development neurochemistry novel physical conditioning restraint stress sample fixation

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Center for Psychiatric Neuroscience (CPN) examines the neurochemical causes of depression. Of all mental illnesses, depression is the most common disorder and is a serious and persistent medical illness affecting the thoughts, mood, activity, and physical health of 9.9 million American adults in any given year. Integrated studies conducted by CPN investigators use postmortem brain tissue from animals treated chronically with an antidepressant drug or exposed to chronic stress to examine novel hypotheses that may ultimately lead to more effective treatments for this debilitating disease. The CPN and the University of Mississippi Medical Center (UMC) use research animals when the information that will be gained may be useful in saving human lives or treating disease and/or when the information cannot be gained without the use of animals. The CPN and UMC recognize its ethical responsibility to treat animals involved in research humanely, and require that all faculty and staff involved in animal research undergo vigorous training and maintain the highest standards of care. The CPN Animal Core provides brain tissue from animal subjects that have been treated either pharmacologically or behaviorally in a manner that allows CPN investigators to address the specific aims of their individual projects. To allow the assessment of the effects of long-term antidepressant exposure on central nervous system parameters, animals are treated long-term with fluoxetine and sacrificed for postmortem studies of changes in the brain. In addition to the studies with fluoxetine, other animals are exposed to a chronic stress paradigm that results in behavioral symptoms that may be related to human depression. Chronic restraint stress results in alterations in some features of the glutamate and serotonin systems that may well be relevant to the changes detected in these systems in human depression. William L. Woolverton, Ph.D., provides overall supervision of the Animal Core. He is a behavioral pharmacologist with 30 years experience with pharmacological research in behavioral and neurochemical studies. Javier Miguel-Hidalgo, Ph.D., supervises brain dissection, collection and storage. Dr. Miguel-Hidalgo is a neuroanatomist with 18 years experience in dissection, freezing, fixation, storage, and histochemical processing of brain samples in a variety of vertebrate species. Patrick Kyle, Ph.D., will assess fluoxetine levels in blood samples. Dr. Kyle is an analytical toxicologist with 14 years experience in drug analysis and analytical methods development involving GC/MS, HPLC, and LC/MS.

该子项目是利用该技术的众多研究子项目之一资源由 NIH/NCRR 资助的中心拨款提供。子项目和研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金，因此可以在其他 CRISP 条目中表示。列出的机构是对于中心来说，它不一定是研究者的机构。精神病神经科学中心 (CPN) 研究抑郁症的神经化学原因。在所有精神疾病中，抑郁症是最常见的疾病，是一种严重且持续的疾病，每年都会影响 990 万美国成年人的思想、情绪、活动和身体健康。 CPN 研究人员进行的综合研究使用长期接受抗抑郁药物治疗或暴露于慢性压力的动物的死后脑组织来检验新的假设，这些假设最终可能为这种使人衰弱的疾病带来更有效的治疗。当获得的信息可能有助于拯救人类生命或治疗疾病和/或如果不使用动物就无法获得信息时，CPN 和密西西比大学医学中心 (UMC) 就会使用研究动物。 CPN 和 UMC 认识到其人道对待参与研究的动物的道德责任，并要求所有参与动物研究的教职员工接受严格的培训并保持最高标准的护理。 CPN 动物核心提供来自动物受试者的脑组织，这些脑组织经过药理学或行为治疗，使 CPN 研究人员能够实现其个人项目的具体目标。为了评估长期接触抗抑郁药物对中枢神经系统参数的影响，对动物进行长期氟西汀治疗，并处死动物以进行大脑变化的死后研究。除了氟西汀的研究之外，其他动物也暴露于慢性压力范式中，导致可能与人类抑郁症相关的行为症状。慢性束缚压力会导致谷氨酸和血清素系统的某些特征发生变化，这很可能与人类抑郁症中这些系统中检测到的变化有关。 William L. Woolverton 博士负责 Animal Core 的全面监督。他是一位行为药理学家，在行为和神经化学研究方面拥有 30 年的药理学研究经验。 Javier Miguel-Hidalgo 博士负责监督大脑解剖、收集和储存。 Miguel-Hidalgo 博士是一位神经解剖学家，在多种脊椎动物脑样本的解剖、冷冻、固定、储存和组织化学处理方面拥有 18 年经验。帕特里克·凯尔博士将评估血液样本中的氟西汀水平。 Kyle 博士是一位分析毒理学家，在药物分析和分析方法开发（涉及 GC/MS、HPLC 和 LC/MS）方面拥有 14 年经验。