IODOTYROSINE DEHALOGENASE, AN ENZYME REQUIRED FOR IODINE SALVAGE

碘酪氨酸脱卤酶，一种抢救碘所需的酶

• 批准号: 7955175
• 负责人: Nicole A LaRonde
• 金额: $ 0.39万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955175
• 关键词: Active Sites; Binding; Biology; Computer Retrieval of Information on Scientific Projects Database; Cysteine; Enzymes; Flavoproteins; Funding; Grant; Hormones; Institution; Iodide Peroxidase; Iodides; Iodine; Mono-S; Monoiodotyrosine; Recycling; Research; Research Personnel; Resources; Role; Site-Directed Mutagenesis; Source; Structure; Thyroid Hormones; United States National Institutes of Health; deiodination; enzyme mechanism; hormone biosynthesis; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The need for iodide in biology is almost exclusively limited to its role in thyroid hormones. Recycling of thyroidal iodide is critical for maintaining proper hormone levels. The flavoprotein iodotyrosine deiodinase (IYD) salvages iodide from byproducts (mono- and diiodotyrosine, MIT and DIT) of thyroid hormone biosynthesis. The original proposal for the deiodination mechanism of IYD included a nucleophilic attack of the iodo group by an active site cysteine. Although this proposed mechanism has strong precedence, site-directed mutagenesis proved this wrong. Structures of IYD with bound MIT and DIT were sought to elucidate the enzymatic mechanism of this enzyme.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 生物学中碘化物的需求几乎仅限于其在甲状腺激素中的作用。 甲状腺碘化物的回收对于维持适当的激素水平至关重要。 甲状腺激素生物合成的副产物（单和二碘酪氨酸，MIT和DIT）从副产物（单和二豆氨酸，MIT和DIT）碘化碘化物酶（IYD）碘化酶（IYD）的碘化物。 IYD去二化机制的原始建议包括活性位点半胱氨酸对iodo组的亲核攻击。 尽管这种提出的机制具有很强的优先级，但位置定向的诱变证明了这一点。寻求具有绑定的MIT和DIT的IYD结构，以阐明该酶的酶促机理。