MPHR(A): TRANSCRIPTION FACTOR REQUIRED FOR ERYTHROMYCIN RESISTANCE

MPHR(A)：红霉素耐药所需的转录因子

• 批准号: 7955174
• 负责人: Nicole A LaRonde
• 金额: $ 0.39万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955174
• 关键词: Binding; Biological; Computer Retrieval of Information on Scientific Projects Database; DNA; Engineering; Erythromycin; Funding; Gene Expression; Genes; Goals; Grant; Initiator Codon; Institution; Macrolide Antibiotics; Macrolides; Phosphotransferases; Proteins; Research; Research Personnel; Resistance; Resources; Source; Structure; United States National Institutes of Health; genetic regulatory protein; structural biology; transcription factor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The regulatory protein MphR(A) has recently seen extensive use in Synthetic Biological applications such as metabolite sensing and exogenous control of gene expression. This protein negatively regulates the expression of a macrolide 2'-phosphotransferase I resistance gene (mphA) via binding to a 35bp DNA operator upstream of the start codon, and is de-repressed by the presence of erythromycin. Our goal in this project was to determine the difference in structure between the erythromycin bound and free Mphr(A) in order to understand how it might be engineered to respond to different macrolide antibiotics.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 调节蛋白MPHR（A）最近在合成生物学应用中广泛使用，例如代谢产物传感和对基因表达的外源控制。 该蛋白质通过与开始密码子上游的35bp DNA算子结合，对大花环2'-磷酸转移酶I耐药基因（MPHA）的表达进行负调节，并因促红霉素的存在而取消了重复。 我们在该项目中的目标是确定红霉素结合和游离MPHR（a）之间的结构差异，以了解如何对其进行设计以应对不同的大花环抗生素。