IMAGING OF IMMUNOFLUORESCENCE PROBES FOR THE DETECTION OF DIFFERENT DNA DAMAGE

用于检测不同 DNA 损伤的免疫荧光探针成像

• 批准号: 7954888
• 负责人: TATIANA B KRASIEVA
• 金额: $ 0.3万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954888
• 关键词: Area; Biotechnology; Cell Nucleus; Chromosomes; Computer Retrieval of Information on Scientific Projects Database; DNA Damage; DNA Repair; Funding; Grant; Image; Immunofluorescence Immunologic; Institution; Interphase Cell; Lasers; Research; Research Personnel; Resources; Site; Source; Staining method; Stains; United States National Institutes of Health; in vivo; repaired

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Previously it was shown that when the nucleus of interphase cells were irradiated with the NIR laser a darkening occured at the area of laser exposure and that DNA damage repair factors were observed to localize to the area of damage. Currently we are irradiating chromosomes in vivo and have observed a black dot forming at the site of exposure. Immunofluorescence staining will show wether the black dot coincides with the recruitment of DNA damage recognition and repair factors.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 以前表明，当用NIR激光辐射相间细胞的核时，激光暴露区域会发生变暗，并且观察到DNA损伤修复因子定位于损伤区域。 目前，我们正在体内照射染色体，并观察到在暴露部位形成的黑点。免疫荧光染色将表明黑点与DNA损伤识别和修复因子的募集一致。