IMPACT OF HYPERTONIC-HYPERONCOTIC SALINE SOLUTIONS ON ISCHEMIA-REPERFUSION

高渗-高渗盐水溶液对缺血再灌注的影响

• 批准号: 7954884
• 负责人: ANTHONY J DURKIN
• 金额: $ 0.3万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954884
• 关键词: Animals; Area; Arteries; Biotechnology; Blood Vessels; Blood flow; Clinical; Computer Retrieval of Information on Scientific Projects Database; Defect; Failure; Funding; Goals; Grant; Hemoglobin; Histocompatibility Testing; Image; Institution; Ischemia; Lasers; Location; Measures; Mechanics; Methods; Modeling; Obstruction; Operative Surgical Procedures; Patients; Rattus; Reconstructive Surgical Procedures; Reperfusion Injury; Reperfusion Therapy; Research; Research Personnel; Research Project Grants; Resources; Saline; Salts; Secondary to; Severities; Site; Solutions; Source; Spectrum Analysis; Surgeon; Surgical Flaps; Systemic disease; Techniques; Testing; Time; Tissues; Trauma; United States National Institutes of Health; Vascular blood supply; Veins; Water; improved; research study; restoration; soft tissue; tissue processing

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Impact of hypertonic-hyperoncotic saline solutions on ischemia-reperfusion injury in pedicled flaps in rats, as measured by Modulated Imaging tissue spectroscopy. Tissue transfer flaps are a method of moving any tissue from a donor area of the body to a recipient site. The reconstructive surgeon employs two major types of tissue transfer flaps, the pedicled tissue transfer flap and the free tissue transfer flap. A pedicle flap consists of soft tissues connected to the donor location only by the artery and vein, which provide the blood supply to the pedicled tissue transfer flap. The tissues of the pedicled flap are moved from the donor site to the recipient site by rotating the soft tissue of the flap on the arteries and veins, thereby allowing the transfer of tissue from one location to another on the body. In a free tissue transfer flap the arteries and veins are divided from the donor site, and re-attached to the recipient site using microsurgical techniques. Improved surgical techniques have resulted in a high percentage of successful tissue transfer flaps for tissue defects after trauma or oncological ablative surgery. However, flap failures still occur and are usually caused by global insufficiency of blood supply secondary to mechanical obstruction of the vessels, systemic disease, or the ischemia-reperfusion (I/R) injury. This I/R injury occurs directly after reestablishment of the blood flow to ischemic tissues, this process occurs to some extent with all free tissue transfer flaps as the flap is transiently ischemic during the period between detachment from the donor site and re-attachment at the recipient site. I/R injury can also occur with pedicle flaps as well if the blood supply to the flap is interrupted due to "kinking" or external compression of the arteries and / or veins to the flap. Severe I/R injury can result in vascular damage within the tissue transfer flap, leading to poor blood flow and further ischemia, which can result in partial or complete flap loss. Partial or complete flap loss can be devastating to patients undergoing reconstructive surgery. Hypertonic-hyperoncotic saline (HHS) solutions are solutions that contain high concentrations of salts. Prior clinical and animal studies have found that the administration of HHS solutions at the time of re-establishment of blood flow to ischemic tissue can lessen the extent and severity of I/R injury. The goal of this specific research project is to test the impact of HHS solutions on the I/R injury after re-establishment of blood flow to an ischemic tissue transfer flap. We hypothesize that these solutions are able to minimize the effects of I/R injury after the restoration of blood flow to ischemic tissues and thus increase overall flap survival. We believe from our prior experiments and results using hypertonic saline in a rat free flap model that this is the case. This experiment attempts to measure the improved microvascular blood flow by using Modulated Imaging (MI) Spectroscopy. We plan to do so by using MI spectroscopy to demonstrate the changes in total, oxygenated, and deoxygenated hemoglobin as well as the changes in water content within an ischemic flap after reperfusion when treated with hypertonic saline.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 通过调制成像组织光谱法测量，高渗 - 混合盐盐溶液对大鼠缺血 - 再灌注损伤的影响。 组织转移皮瓣是一种将任何组织从人体的供体区域移动到受体部位的方法。 重建外科医生采用了两种主要类型的组织转移皮瓣，编织的组织转移皮瓣和自由组织转移皮瓣。 椎弓根皮瓣由仅由动脉和静脉连接到供体位置的软组织，这些软组织为适应的组织转移瓣提供血液供应。 通过旋转动脉和静脉上的皮瓣的软组织，从供体部位移动到接受者部位，从而将组织从一个位置转移到人体上的另一个位置，从而将其移动到受体部位。 在自由组织转移皮瓣中，动脉和静脉与供体部位分开，并使用微神经​​技术重新连接到受体部位。 改进的手术技术已导致创伤或肿瘤消融手术后的组织缺陷成功的组织不足的成功组织转移皮瓣。但是，皮瓣衰竭仍会发生，通常是由于血管，全身性疾病或缺血性灌注（I/R）损伤的全球血液供应不足引起的。这种I/R损伤直接发生在血液流动到缺血组织后，在某种程度上发生了所有自由组织转移皮瓣，因为在从捐助者部位脱离的期间与受体部位的重新跟踪之间，该襟翼是短暂的缺血性。 如果由于“扭结”或动脉的外部压缩和 /或静脉向皮瓣的外部压缩，椎弓根瓣也可能发生I / R损伤。 严重的I/R损伤会导致组织转移皮瓣内的血管损伤，导致血液流量差和进一步的缺血，从而导致部分或完全的皮瓣损失。 部分或完全的皮瓣损失可能是对接受重建手术的患者造成的。 高渗 - 混血盐水（HHS）溶液是含有高浓度盐的溶液。 先前的临床和动物研究发现，在重新建立血液流动到缺血组织时的HHS溶液可以减轻I/R损伤的严重程度和严重程度。 该特定研究项目的目的是测试HHS溶液对血液流动重建为缺血组织转移瓣后I/R损伤的影响。我们假设这些溶液能够最大程度地减少血液流动到缺血组织后I/R损伤的影响，从而增加整体皮瓣存活。 我们相信，从大鼠自由皮瓣模型中使用高渗盐水的实验和结果是这种情况。 该实验试图通过使用调制成像（MI）光谱法测量改善的微血管血流。 我们计划通过使用MI光谱法证明总体，氧合和去氧血红蛋白的总变化，以及用催眠盐水治疗后，再灌注后缺血性瓣中的水含量变化。