STRUCTURE OF THE TWO-PARTNER SECRETION PROTEIN SHLB FROM SERRATIA MARCESCENS

粘质沙雷氏菌的双伙伴分泌蛋白 SHLB 的结构

• 批准号: 7957251
• 负责人: Laura N. Vandenberg
• 金额: $ 0.52万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957251
• 关键词: Biogenesis; Complex; Computer Retrieval of Information on Scientific Projects Database; Crystallography; Eukaryota; Family; Family member; Funding; Gram-Negative Bacteria; Grant; Institution; Light; Membrane; Mitochondria; Names; Pathway interactions; Process; Protein Secretion; Proteins; Research; Research Personnel; Resources; Serratia marcescens; Source; Structure; Synchrotrons; Transport Process; United States National Institutes of Health; Virulence; periplasm

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Many large virulence proteins of pathogenic gram-negative bacteria are secreted by the Two-partner secretion (TPS) pathway. As the name implies, only two proteins are required for this process: the secreted exoprotein (TpsA) and the ¿}¿¿-barrel transport channel in the OM (TpsB). After crossing the inner membrane via the SecY complex, TpsA is likely translocated across the OM in an unfolded state. Many aspects of the transport process are unclear: how is periplasmic TpsA kept soluble, how does targeting to the OM channel occur, and how is TpsA transported through the TpsB channel, given that no external energy is required? To begin answer these questions we plan to crystallize a TpsB channel. The TpsB proteins are distantly related to the Omp85 family, which are involved in the biogenesis of OM proteins. Members of this family are also present in eukaryotes (mitochondria). Both for TPS and Omp85 family members no structural information is known at present.

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 两伴侣分泌（TPS）途径分泌许多致病革兰氏阴性细菌的大型病毒蛋白。顾名思义，此过程仅需要两种蛋白质：分泌的异蛋白（TPSA）和OM（TPSB）中的荷桶传输通道。通过SECY复合物越过内膜后，TPSA可能以展开状态在OM上翻译。运输过程的许多方面尚不清楚：鉴于不需要外部能量，tpsa如何保持稳固，如何靶向OM通道，如何通过TPSB通道运输TPSA？为了开始回答这些问题，我们计划结晶tpsb频道。 TPSB蛋白与Omp85家族特别相关，这些家族与OM蛋白的生物发生有关。这个家庭的成员也存在于真核生物（线粒体）中。目前，对于TPS和OMP85家族成员，尚无结构信息。