IMMUNE RESPONSES TO MICROSPORIDIA

对小孢子虫的免疫反应

基本信息

批准号：
7958588
负责人：
Elizabeth Schmidt Didier
金额：
$ 6.05万
依托单位：
TULANE UNIVERSITY OF LOUISIANA
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-05-01 至 2010-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7958588
关键词：
Algorithms Animals Cells Complementary DNA Computer Retrieval of Information on Scientific Projects Database Computer software Data Data Set Diarrhea Disease Elements Enterocytozoon bieneusi Exhibits Feces Funding Genes Genetic Transcription Grant Hepatic Histocytochemistry Hour Housing Human Image Immune Immune response Incubated Infection Inflammatory Institution Interferon Type II Interferons Intestines Label Lymphocyte Macaca Macaca mulatta Macaca nemestrina Measurement Microarray Analysis Microsporidia Monkeys Oligonucleotides Papio Pathway Analysis Pathway interactions Primates Printing Publishing RNA Reporting Reproduction spores Research Research Personnel Resources Sampling Signal Transduction Slide Source Technology Tumor Necrosis Factor-alpha United States National Institutes of Health base cytokine human TNF protein nonhuman primate pathogen response wasting

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Enterocytozoon bieneusi is the most prevalent microsporidian infecting humans, yet virtually no studies have been published about the immune responses to this pathogen. Nonhuman primates are natural hosts of E. bieneusi infections, and na¿ve and immune-compromised rhesus macaques exhibit similar signs of disease (eg. diarrhea and wasting) as reported in immune-compromised infected humans. E. bieneusi infections are being reported more frequently in immune-competent humans, and 35-40% of the immune-competent pigtail macaques, rhesus macaques, and baboons housed outdoors at the Tulane National Primate Research Center were detected with E. bieneusi spores in feces by histochemistry and PCR. To begin to study the immune responses to this microsporidian, a preliminary microarray analysis was performed. Duodenal and jejunal intestinal cells from immune-competent E. bieneusi-infected and non-infected rhesus macaques (with and without diarrhea) were incubated with E. bieneusi spores or medium for 24 hours. Total RNA was extracted to generate Cy-labeled cDNA samples using the Low RNA Input Linear Amplification Kit (Agilent Technologies Inc., CA). Labeled cDNA was hybridized overnight to the 44,000 element 60mer oligonucleotide based rhesus macaque microarray printed in a 4x44k format (Agilent Technologies Inc., CA), which can interrogate the transcription of over 18,000 unique macaque genes at once. After hybridization and washing, slides were evaluated in a dual-confocal continuous microarray scanner (GenePix 4000B) using GenePix Pro version 6.1 as the image acquisition and extraction software. Microarray data were based on duplicate measurements. Pathway analyses were performed by uploading significant data sets into Ingenuity Pathways Analysis algorithm. Among the pathways and cytokines related to immune responses that were significantly perturbed (P 0.05) in the E. bieneusi-infected monkey lymphocytes with diarrhea and the uninfected monkeys were those related to inflammatory disease, hepatic disease, and interferon signalling (e.g. TNF-alpha, IFN-gamma). These pathways were not significantly perturbed in intestinal cells of infected animals that were not exhibiting signs of diarrhea, suggesting that healthy immune-competent animals are able to regulate inflammatory pathways in response to E. bieneusi infection.

该副本是利用众多研究子项目之一由NIH/NCRR资助的中央赠款提供的资源调查员（PI）可能已经从其他NIH来源获得了主要资金，因此，在列出的机构中可能会被压制对于中心，这不一定是调查员的工厂。肠肠肠Bieneusi是最普遍的微孢子虫感染人类，但虚拟的没有发出的泡沫，使ablishet免疫对病原体，而非人类的灵长类动物是na e。 VE和免疫受损的恒河猴表现出相似的疾病迹象（例如，E. Bieneusi感染的人在免疫能力的人类中更多地报道了Bieneusi感染，而35-40％的免疫能力的Pigtail猕猴和户外户外的babo型则更多。通过组织化学和PCR检测到粪便中的Tulane National Primate Central在粪便中进行了生物孢子。。 Inc.，CA可以使用Genepix Pro 6.1作为图像和萃取软件来审问超过18,000个独特的猕猴的转录。在E. bieneusi感染的猴子菌淋巴细胞中显着扰动（p 0.05）和未感染的猴子是与感染动物的肠道细胞扰动的那些与炎性疾病有关响应生物息肉感染的炎症途径。