GASTROINTESTINAL DISEASE IN CAPTIVE RHESUS MACAQUES

圈养恒河猴的胃肠道疾病

• 批准号: 8358108
• 负责人: Elizabeth Schmidt Didier
• 金额: $ 3.72万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358108
• 关键词: Age; Amino Acid Motifs; Animal Hospitals; Animal Housing; Animals; Antibodies; Binding; Calicivirus; Clinic; Cross-Sectional Studies; Diagnostic; Diarrhea; Enteral; Enterocytozoon bieneusi; Feces; Funding; Gastrointestinal Diseases; Gender; Genetic; Grant; High Prevalence; Human; Macaca; Macaca mulatta; Microsporidia; Morbidity - disease rate; National Center for Research Resources; Norovirus; Phylogenetic Analysis; Prevalence; Primates; Principal Investigator; RNA-Directed RNA Polymerase; Reporting; Research; Research Infrastructure; Resources; Sampling; Sapovirus; Serum; Source; Specimen; Testing; United States National Institutes of Health; Virus; cost; neutralizing antibody; nonhuman primate; transmission process

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Persistent diarrhea is one of the leading causes of morbidity in captive nonhuman primates (NHPs). During the current reporting period, a cross-sectional study was continued to test fecal specimens obtained from rhesus macaques admitted to the veterinary clinics for diarrhea and/or wounding as well as from animals housed in field cages at the Tulane National Primate Research Center (TNPRC) for microsporidia shedding using PCR diagnostics. Of 194 clinic specimens analyzed, 28 of 95 (29.5%) were positive for microsporidia and of 99 field specimens analyzed, 35 of 99 (35.4%) were positive for microsporidia with an overall prevalence of 32.5%. Fisher's Exact test indicated no significant differences in prevalence associated with gender, age, or clinic vs field animals. Enterocytozoon bieneusi was by far, the most common species identified (54 of 64; 84.3 %). The objective of this study is also to determine the prevalence of anti-norovirus (NoV), sapovirus (SaV) and Tulane calicivirus (TV) antibodies in rhesus macaques of the TNPRC and evaluate the antigenic relationship between these viruses. So far, we tested 515 rhesus macaques for this purpose. A high prevalence of NoV (51-61%) and SaV (50-56%) binding antibodies and TV (69%) neutralizing antibodies were detected. The high prevalence of human and rhesus CV-specific serum antibodies suggests the frequent exposure of colony macaques to enteric CVs including the possibility of CV transmission between human and NHP hosts. More recently, 325 randomly selected stool samples collected from TNPRC rhesus macaques were tested using modified degenerate primers targeting conserved amino acid motifs in the calicivirus RNA dependent RNA polymerases (RdRp). Thirty-six (11%) of the 325 stools samples tested yielded TV specific sequences, confirming that TVs are endemic in the TNPRC. According to phylogenetic analysis, the 36 TV isolates can be classified into two genogroups and at least four genetic types.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 持续性腹泻是圈养非人类灵长类动物（NHP）发病的主要原因之一。 在当前的报告期间，继续一项横断面研究，测试从腹泻和/或受伤的兽医诊所获得的恒河猕猴，以及从杜兰国家灵长类动物研究中心（TNPRC）的田间笼子中的动物中获得的，用于使用PCR诊断的菌皮降低。 在分析的194个诊所标本中，有95个中的28个（29.5％）为微孢子虫阳性，在分析的99个野外样品中，99个中的35个（35.4％）为阳性，阳性率为32.5％，总体患病率为32.5％。 费舍尔的精确检验表明，与性别，年龄或诊所与野外动物有关的患病率没有显着差异。 到目前为止，肠球菌生物肠肠菌是最常见的物种（64个中的54个； 84.3％）。 这项研究的目的还是确定抗螺旋病毒（NOV），SAPOVIRUS（SAV）和TULANE CALICIVIRUS（TV）抗体中TNPRC的抗体的患病率，并评估这些病毒之间的抗原关系。到目前为止，我们为此目的测试了515个恒河猕猴。检测到了11月（51-61％）和SAV（50-56％）结合抗体和TV（69％）中和抗体的高患病率。人类和恒河神特异性的血清抗体的高流行率表明，猕猴经常暴露于肠中CV，包括人与NHP宿主之间CV传播的可能性。 最近，使用修饰的退化引物靶向钙病毒RNA依赖性RNA聚合酶（RDRP）中的325个从TNPRC恒河猕猴中收集的随机粪便样品。在经过测试的325个凳子样品中，有36个（11％）产生了特定的电视序列，证实了电视在TNPRC中是特有的。根据系统发育分析，可以将36个电视分离株分为两种基因组和至少四种遗传类型。