Simultaneous Stimulation and Recording in Scalable Microelectrode Arrays

可扩展微电极阵列中的同步刺激和记录

• 批准号: 8058252
• 负责人: James Ross
• 金额: $ 53.22万
• 依托单位: AXION BIOSYSTEMS, LLC
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-15 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8058252
• 关键词: Simultaneous; Stimulation; Recording; Scalable; Microelectrode

DESCRIPTION (provided by applicant): The proposed study utilizes novel electronics and microfabrication techniques to create scalable, in-vitro Microelectrode Array (MEA) technologies that conform to industry standards for multiwell plates. This research will not only enable rapid advancements in the study of network-level electrophysiology, but it will also create new opportunities for pharmaceutical research and toxicity screening. This Phase II proposal involves two significant developments for neurological research. Specifically, Aim 1 builds on the scalable simultaneous stimulation and recording Integrated Circuit (IC) developed in Phase I to produce a full electronics platform for capturing, processing and storing electrophysiological information. Among its many advantages, this electronics platform will recover signals traditionally obscured by stimulation artifacts. This captured data, combined with the ability to simultaneously manage 768 microelectrodes and automate experimental protocols, will provide new measures of single-cell and network-level neural activity. Aim 2 will produce scalable, inexpensive, and flexible processes for fabricating multiwell MEAs that, in conjunction with the electronics developed in Aim 1, will yield a high-throughput, network-electrophysiology toolset. PUBLIC HEALTH RELEVANCE: This research uses novel electronic and fabrication technologies to create faster, lower-cost methods for neural research. Ultimately, this development will facilitate medical and scientific discoveries that will benefit the treatment of neural disorders such as Parkinson's disease and epilepsy.

描述（由申请人提供）：拟议的研究利用新颖的电子和微加工技术来创建可扩展的，即使微电极阵列（MEA）技术，该技术符合符合Multiwell Plates行业标准的技术。这项研究不仅将在网络级电生理学研究中迅速发展，而且还将为药物研究和毒性筛查创造新的机会。该第二阶段的建议涉及神经研究的两个重大发展。具体而言，AIM 1建立在I阶段在I期中开发的可扩展刺激和记录集成电路（IC）上，以生成一个完整的电子平台，用于捕获，处理和存储电生理信息。在其许多优势中，这个电子平台将恢复传统上被刺激工件掩盖的信号。该捕获的数据，结合了同时管理768个微电极并自动化实验协议的能力，将提供单细胞和网络级神经活动的新度量。 AIM 2将产生可扩展，廉价且灵活的工艺，用于制造多井度量，并结合在AIM 1中开发的电子设备，将产生高通用的网络电子生理学工具集。 公共卫生相关性：这项研究使用新颖的电子和制造技术来为神经研究创建更快，低成本的方法。最终，这一发展将促进医学和科学发现，从而有助于帕金森氏病和癫痫等神经疾病的治疗。