IMPLICATIONS OF DISULFIDES BREAKING SEQUENCE IN REDUCTIVE UNFOLDING PATHWAYS

二硫化物断裂序列对还原性展开路径的影响

• 批准号: 7721227
• 负责人: JUN WANG
• 金额: $ 0.2万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-15 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721227
• 关键词: Bos taurus; Cattle; Computer Retrieval of Information on Scientific Projects Database; Crystallography; Disulfides; Funding; Grant; Institution; Methods; Pancreatic ribonuclease; Pathway interactions; Proteins; Research; Research Personnel; Resources; Source; Standards of Weights and Measures; Structure; Time; United States National Institutes of Health; disulfide bond; irradiation; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The sequence of the breakage of disulfide bonds due to x-ray irradiation was observed by time-dependent crystallographic studies of proteins using the strategy we developed for time-dependent structure observations on the atomic scale in a protein crystal for standard crystallography experiments. These observed sequences are related to the reductive unfolding pathways in proteins. he current study focuses on bovine pancreatic ribonuclease A, where the observed breaking sequence is being compared to results by other established methods.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 通过对蛋白质的时间依赖性晶体学研究观察到蛋白质的二硫键断裂序列，使用我们为标准晶体学实验的蛋白质晶体中的时间依赖性结构观测而开发的策略。 这些观察到的序列与蛋白质的还原性展开途径有关。 他目前的研究重点是牛胰腺核糖核酸酶A，其中观察到的断裂序列正在与其他既定方法进行比较。