HIGH RESOLUTION STRUCTURES BY MINIMIZING RADIATION EFFECTS

通过最小化辐射效应实现高分辨率结构

• 批准号: 7369519
• 负责人: JUN WANG
• 金额: $ 0.02万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7369519
• 关键词: RESOLUTION; STRUCTURES; MINIMIZING; RADIATION; EFFECTS; RESOLUTION; STRUCTURES; MINIMIZING; RADIATION; EFFECTS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Radiation damage on protein crystals, especially since data is collected at third generation synchrotron facilities, is an issue and is getting more and more attention. Ultra high resolution structures are what people aspire to obtain. However, ultra high resolution structures are most sensitive to and affected by radiation damage. We applied the new strategy we developed for time-dependent structure observations to remove or minimize radiation effects and thus yield the real high resolution structure from a standard dataset.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。蛋白质晶体的辐射损伤，尤其是因为数据是在第三代同步设备中收集的，这是一个问题，并且正在引起越来越多的关注。超高分辨率结构是人们渴望获得的。但是，超高分辨率结构对辐射损伤最敏感和影响。我们将开发的新策略应用于时间依赖性结构观测值，以消除或最小化辐射效应，从而从标准数据集中产生实际的高分辨率结构。