Colorado Gene Array Core

科罗拉多基因阵列核心

基本信息

批准号：
7291104
负责人：
BORIS TABAKOFF
金额：
$ 46万
依托单位：
UNIVERSITY OF COLORADO DENVER
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-09-30 至 2011-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7291104
关键词：
Alcohol consumption Alcoholism Alcohols Anxiety Atlases Behavioral Boutos Brain Brain region Candidate Disease Gene Chronic Collaborations Colorado Complex Control Locus Correlation Studies Data Data Analyses Databases Development Ethanol Female Gene Expression Gene Expression Profile Gene Expression Profiling Generations Genes Genome Genomics Genotype Goals Indiana Informatics Knockout Mice Letters Maps Methodology Methods Microarray Analysis Microsatellite Repeats Modeling Molecular Profiling Mouse Strains Mus Oligonucleotide Microarrays Phenotype Polymerase Chain Reaction Procedures Proteome Proteomics Quantitative Trait Loci RNA Interference Rat Strains Rattus Reagent Recombinants Reporting Research Personnel Resources Reverse Transcriptase Polymerase Chain Reaction SNP genotyping Tennessee Testing Transgenic Organisms Universities Validation Withdrawal Work alcohol behavior base behavior measurement drinking drinking behavior male novel progenitor tool trait

项目摘要

DESCRIPTION (provided by applicant): The Colorado Gene Array Core will focus on the creation of databases that can be used for developing expression QTL (eQTL) maps for mouse and rat brain. We are currently expanding the database of brain gene expression profiles for males and females of the BxD Rl mouse strains. The proposed studies will generate a similar database of gene expression profiles from brains of 31 Rl strains of BxH/HxB rats and the progenitor strains (males and females separately), and microsatellite and SNP genotyping of the rat strains will be performed in collaboration with Dr. Rob Williams, University of Tennessee. This information will allow for eQTL mapping for rat brain gene expression as well as mapping of QTLs for alcohol drinking in the rat strains. When combined with QTL analysis of complex traits, the generation of eQTLs facilitates the identification of candidate genes for these traits, and the Core will use this procedure and the generated data to identify candidate genes that contribute to anxiety and other alcohol related behaviors in the rats. The availability of brain gene expression data and eQTL mapping of the mouse and rat brain transcriptome will produce a novel and unique resource for use by alcoholism investigators. The Core will also perform gene expression analysis and quantitative RT-PCR for collaborating INIA investigators, and provide access to methodology for normalization, statistical analysis and eQTL searches of the generated data.

描述（由申请人提供）：科罗拉多基因阵列核心将专注于创建可用于开发小鼠和大鼠大脑表达 QTL (eQTL) 图谱的数据库。我们目前正在扩大 BxD R1 小鼠品系雄性和雌性的大脑基因表达谱数据库。拟议的研究将生成一个类似的数据库，其中包含 31 只 Rl 品系 BxH/HxB 大鼠及其祖细胞品系（分别为雄性和雌性）的大脑基因表达谱，并且将与 Dr. 合作对大鼠品系进行微卫星和 SNP 基因分型。罗布·威廉姆斯，田纳西大学。这些信息将有助于绘制大鼠大脑基因表达的 eQTL 图谱，以及绘制大鼠品系中饮酒的 QTL 图谱。当与复杂性状的 QTL 分析相结合时，eQTL 的生成有助于识别这些性状的候选基因，并且 Core 将使用此程序和生成的数据来识别导致大鼠焦虑和其他酒精相关行为的候选基因。小鼠和大鼠大脑转录组的大脑基因表达数据和 eQTL 绘图的可用性将为酗酒调查人员提供一种新颖且独特的资源。该核心还将为合作的 INIA 研究人员进行基因表达分析和定量 RT-PCR，并提供对所生成数据进行标准化、统计分析和 eQTL 搜索的方法。