Gene Array Technology Center for Alcohol Research (The R-GAP)

酒精研究基因阵列技术中心 (R-GAP)

• 批准号: 7262931
• 负责人: BORIS TABAKOFF
• 金额: $ 89.48万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7262931
• 关键词: Gene; Array; Technology; Center; Alcohol

DESCRIPTION (provided by applicant): The Rodent Gene Array Program (R-GAP) will focus on the creation of databases that can be used for developing expression QTL (eQTL) maps for mouse and rat brain and for use in extensive studies of expression control elements. The proposed studies will generate a database of gene expression profiles from brains of 75 Rl strains of ISSXILS mice (LXS mice; males and females separately), and integrate ultrahigh density SNP genotyping of the mouse strains. Additionally, in collaboration with the Gene Network, a web-based resource for gene expression analysis, all of our key data for LXS strains will be curated and made available for public use. These valuable new datasets will catalyze eQTL mapping, as well as mapping of QTLs for alcohol-related behaviors currently being acquired in the LXS set. When combined with QTL analysis of complex traits, the generation of eQTLs facilitates the identification of candidate genes for these traits, and R-GAP will exploit this strategy to identify strong candidate genes that contribute to anxiety and alcohol related behaviors in LXS mice, as well as in other strains of mice and rats. The availability of brain gene expression data and eQTL mapping of brain transcriptome will produce a novel and unique resource for use by alcoholism investigators. The data on gene expression which we will derive from large panels of inbred mice will complement data generated from the Rl strains of mice, and is structured to take advantage of whole genome sequencing data being generated by the NIEHS Mouse Resequencing Project. The RGAP database will also feature an integrated neuroinformatics component that provides information and methods that can be used to search for 5' transcriptional control modules that interact with transcription factors located within the eQTLs and epistatic loci. The R-GAP will not only generate data valuable to alcoholism investigators, but it will also provide access to methodology for normalization, statistical analysis and eQTL searches of the generated data. R-GAP will, in essence, act as the "back end" of a data generating and initial screening component which will be used with the GeneNetwork. GeneNetwork will generate the "front end" features to make all data available to the research community at large.

描述（由申请人提供）：啮齿动物基因阵列计划（R-GAP）将专注于创建数据库，该数据库可用于开发小鼠和大鼠大脑的表达 QTL（eQTL）图谱，并用于表达控制的广泛研究元素。拟议的研究将生成 75 个 R1 品系 ISSXILS 小鼠（LXS 小鼠；分别为雄性和雌性）大脑基因表达谱的数据库，并整合小鼠品系的超高密度 SNP 基因分型。此外，通过与用于基因表达分析的网络资源 Gene Network 合作，我们将整理 LXS 菌株的所有关键数据并供公众使用。这些有价值的新数据集将促进 eQTL 定位，以及当前在 LXS 集中获取的酒精相关行为的 QTL 定位。当与复杂性状的 QTL 分析相结合时，eQTL 的生成有助于识别这些性状的候选基因，R-GAP 将利用这一策略来识别导致 LXS 小鼠焦虑和酒精相关行为的强候选基因。与其他品系的小鼠和大鼠一样。大脑基因表达数据和大脑转录组 eQTL 作图的可用性将为酗酒研究人员提供一种新颖且独特的资源。我们将从大型近交小鼠组中获得的基因表达数据将补充从 R1 品系小鼠生成的数据，并且被构造为利用 NIEHS 小鼠重测序项目生成的全基因组测序数据。 RGAP 数据库还将具有集成的神经信息学组件，该组件提供可用于搜索与 eQTL 和上位基因座内的转录因子相互作用的 5' 转录控制模块的信息和方法。 R-GAP 不仅会生成对酗酒调查人员有价值的数据，而且还将提供对所生成数据进行标准化、统计分析和 eQTL 搜索的方法。 R-GAP 本质上将充当数据生成和初始筛选组件的“后端”，该组件将与 GeneNetwork 一起使用。 GeneNetwork 将生成“前端”功能，以使所有数据可供整个研究界使用。