Gene Array Technology Center for Alcohol Research

酒精研究基因阵列技术中心

• 批准号: 6641211
• 负责人: BORIS TABAKOFF
• 金额: $ 146.06万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6641211
• 关键词: DNA; Internet; RNA; alcohols; biomedical facility; biotechnology; brain; ethanol; gene environment interaction; gene expression; genetic transcription; genotype; informatics; information dissemination; laboratory mouse; liver; microarray technology; molecular biology information system; nucleic acid quantitation /detection; nucleic acid sequence; tissue /cell culture; training; workshop

APPLICANT?S ABSTRACT: The aim of this proposal is to establish a gene array technology core facility (the Gene Array Technology Center for Alcohol Research, GATC) to serve as a national resource for alcohol research. The collective goal of the GATC is to provide the technology base to allow investigators to delineate the gene expression profiles for ethanol-induced changes in transcriptional responses and to delineate inherent differences in gene expression profiles in animals (including humans) which display a particular type of response to ethanol. The GATC will format and print on glass slides a standardized set of gene arrays for human, mouse, and rat. These arrays will be available to NIAAA investigators for use in their alcohol- related research. The GATC will be an invaluable resource that will provide all NIAAA investigators access to gene expression discovery technologies. The GATC website will provide detailed protocols for isolation of RNA, preparation of fluorescently-labeled target probes, and hybridization procedures. The website will be interactive for GATC consultation- with investigators having queries regarding experimental procedures, quality control, and troubleshooting. For investigators that do not have array scanner access, hybridized slides may be sent back to the GATC, where they will be analyzed. The data will be returned to the investigator in spreadsheet form. The GATC bioinfonnaties group will work with NIAAA investigators for analysis of their gene expression data. The bioinformatics group will also develop a highly integrated database that includes gene expression profile data with DNA sequence from experiment protocols involving ethanol- induced changes in transcriptional responses and differences in gene expression profiles inherent to specific responses to ethanol. When NLAAA investigators publish their gene expression studies, the findings will be integrated into the NIAAA gene expression database for alcohol research. The GATC is also prepared to provide education and training for gene array technology, bioinformatics, and database management. A "visiting scientist" program will be established where small groups of 3-10 NIAAA investigators will work in the GATC during intensive three-day workshops. Finally, the GATC will define normal gene expression patterns in specific regions of the brain and the liver, two primary tissues affected by alcohol. To understand "abnormal" gene expression in alcohol responses, "normal" gene expression must be defined. ne analysis of normal gene expression will be a major database resource for NLKAA investigators. Cumulatively, the GATC will provide the gene arrays, informatics power, and database management for gene expression discovery that will form the basis for new hypotheses in alcohol research.

申请人摘要： 本提案的目的是建立基因阵列技术核心设施 （酒精研究基因阵列技术中心，GATC） 国家酒精研究资源。 GATC 的集体目标是 为研究人员描绘基因提供技术基础 乙醇诱导的转录反应变化的表达谱 并描绘动物基因表达谱的内在差异 （包括人类）对乙醇表现出特定类型的反应。这 GATC 将格式化一组标准化基因阵列并将其打印在载玻片上 适用于人类、小鼠和大鼠。这些阵列将提供给 NIAAA 研究人员用于酒精相关研究。 GATC 将是 宝贵的资源，将为所有 NIAAA 研究人员提供获取基因的途径 表达发现技术。 GATC 网站将提供详细信息 RNA 分离、荧光标记靶标制备的方案 探针和杂交程序。该网站将为 GATC 提供互动功能 咨询——与对实验有疑问的研究人员进行咨询 程序、质量控制和故障排除。对于调查人员来说 没有阵列扫描仪访问权限，混合载玻片可能会被发送回 GATC， 将在哪里对它们进行分析。数据将返回给调查员 电子表格形式。 GATC 生物信息小组将与 NIAAA 合作 研究人员分析他们的基因表达数据。生物信息学 集团还将开发一个高度集成的数据库，其中包括基因 表达谱数据以及来自实验方案的 DNA 序列，涉及 乙醇诱导的转录反应变化和基因差异 对乙醇的特异性反应所固有的表达谱。当NLAAA 研究人员发表他们的基因表达研究，结果将 整合到 NIAAA 基因表达数据库中进行酒精研究。这 GATC还准备提供基因芯片方面的教育和培训 技术、生物信息学和数据库管理。一位“访问科学家” 将建立由 3-10 名 NIAAA 调查员组成的小组 将在 GATC 参加为期三天的密集研讨会。最后，GATC 将定义大脑特定区域的正常基因表达模式 和肝脏，这是受酒精影响的两个主要组织。要了解 酒精反应中“异常”基因表达，“正常”基因表达必须 被定义。对正常基因表达的分析将成为一个主要数据库 NLKAA 调查员的资源。 GATC 将累计提供该基因 用于基因表达的阵列、信息学能力和数据库管理 这一发现将成为酒精研究新假设的基础。