FLUORESCENCE MICROSCOPY OF PROTEINS INVOLVED IN GOLGI-TO-VACUOLE VESICLE TRAFFIC

参与高尔基体到液泡囊泡运输的蛋白质的荧光显微镜

• 批准号: 7723724
• 负责人: Alexey Jarrell Merz
• 金额: $ 1.95万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7723724
• 关键词: Coated vesicle; Complex; Computer Retrieval of Information on Scientific Projects Database; Fluorescence Microscopy; Funding; Goals; Golgi Apparatus; Grant; In Vitro; Institution; Pathway interactions; Proteins; Research; Research Personnel; Resources; Role; Source; United States National Institutes of Health; Vacuole; Vesicle; in vivo; insight; interest; trafficking; vesicle/vacuole

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are interested in the role of known vacuole fusion factors within the AP-3 vesicle trafficking pathway. While we have identified in vitro interactions between the AP-3 vesicle coat complex and a subset of fusion factors, whether these interactions occur in vivo remains unknown. Our goal is to use fluorescence microscopy to determine the colocalization of AP-3 with these fusion factors in vivo and at which cellular compartment this may take place. This information will provide insight into whether AP-3 interacts with the fusion factors in vivo and the possible function of these interactions.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 我们对AP-3囊泡运输途径中已知的液泡融合因子的作用感兴趣。虽然我们已经确定了AP-3囊泡涂层复合物与融合因子的一部分之间的体外相互作用，但这些相互作用是否在体内出现仍然未知。我们的目标是使用荧光显微镜确定AP-3与体内这些融合因子的共定位以及可能发生的细胞隔室。该信息将提供有关AP-3是否与体内融合因子相互作用以及这些相互作用的可能功能的洞察力。