PROTEIN COMPOSITION OF ACTIN TAILS ASSEMBLED IN YEAST EXTRACTS

酵母提取物中组装的肌动蛋白尾部的蛋白质组成

• 批准号: 7957840
• 负责人: DAVID G DRUBIN
• 金额: $ 0.33万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957840
• 关键词: Actins; Biology; Cells; Clathrin; Complex; Computer Retrieval of Information on Scientific Projects Database; Cytosol; Defect; Disease; Endocytosis; Environment; Funding; Fungal Genome; Grant; Institution; Laboratories; Life; Mass Spectrum Analysis; Mediating; Membrane; Microfilaments; Microspheres; Molecular; Polystyrenes; Process; Proteins; Reaction; Research; Research Personnel; Resources; Role; Saccharomyces cerevisiae; Saccharomycetales; Set protein; Source; Structure; System; Tail; United States National Institutes of Health; Vesicle; Yeasts; base; coated pit; extracellular; in vivo; macromolecule

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Clathrin-mediated endocytosis is a fundamental process in living cells, allowing them to capture macromolecules efficiently from the extracellular environment and package them into vesicles in the cytosol. Numerous diseases can be traced to a defect in the succession of molecular reactions leading to endocytosis. In the budding yeast Saccharomyces cerevisiae, endocytosis is localized to cortical actin patches, where a dense network of actin filaments is polymerized around clathrin-coated pits. This network is regulated by a complex set of proteins which cooperate in order to generate membrane invagination. We have recently developed in our laboratory a system in yeast extracts for the assembly of actin based structures around polystyrene microbeads. We believe that the protein composition of these structures could be similar to the protein composition found in vivo in cortical actin patches. We isolated the beads with their associated actin structures, and we would like to determine the protein composition by using mass spectroscopy. These results should allow us to further analyze the role of the different components during endocytosis.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 网格蛋白介导的内吞作用是活细胞中的一个基本过程，使它们可以从细胞外环境中有效地捕获大分子，并将其包装成细胞质中的囊泡。许多疾病可以追溯到导致内吞作用的分子反应连续的缺陷。在酿酒酵母的萌芽酵母菌中，内吞作用定位于皮质肌动蛋白斑块，其中蛋白丝丝的致密网络围绕涂有网状蛋白涂层的凹坑进行聚合。该网络受一组复杂的蛋白质的调节，这些蛋白质合作以产生膜不足。 我们最近在实验室中开发了酵母提取物中的系统，用于围绕聚苯乙烯微粒的基于肌动蛋白的结构组装。我们认为，这些结构的蛋白质组成可能类似于皮质肌动蛋白斑块中体内发现的蛋白质成分。我们及其相关的肌动蛋白结构分离了珠子，我们想通过使用质谱来确定蛋白质组成。这些结果应使我们能够进一步分析内吞作用期间不同成分的作用。