COBRE: UND: MASS SPECTROMETRY CORE FACILITY

COBRE：UND：质谱核心设施

• 批准号: 7720881
• 负责人: Mikhail Y Golovko
• 金额: $ 20.05万
• 依托单位: UNIVERSITY OF NORTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720881
• 关键词: Budgets; Centers of Research Excellence; Chemicals; Commit; Computer Retrieval of Information on Scientific Projects Database; Contract Services; Core Facility; Coupled; Data Analyses; Electrons; Ensure; Equipment; Funding; Gases; Grant; Growth; Institution; Ions; Lipid Peroxidation; Liquid substance; Maintenance; Mass Spectrum Analysis; Methods; National Center for Research Resources; Performance; Procedures; Process; Prostaglandins; Research; Research Personnel; Resolution; Resources; Services; Source; Spectrometry; Spectrometry, Mass, Electrospray Ionization; Standards of Weights and Measures; Support Groups; Techniques; Time; Training; Travel; United States National Institutes of Health; instrument; instrumentation; ionization; mass spectrometer; microbial alkaline proteinase inhibitor; nano; neuroinflammation; repaired

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Mass Spectrometry (MS) Core facility has, for the last five years, supported numerous investigators within and outside our COBRE-supported group. The objective here is to continue providing investigators at UND with a very high level of equipment access and technical support for their ongoing research efforts. The MS Core facility was built and equipped through funding provided by our COBRE I grant and the NCRR. The facility is equipped with three chromatographic/mass spectrometric instruments including (1) a high resolution electrospray-quadrupole/time of flight MS (ESI-QTOF) QSTAR SL equipped with nano-LC, (2) a ESI-triplequadrupole MS API 3000 with high performance liquid chromatograph (LC-ESI-MS/MS), and (3) a gas chromatograph with ion trap mass spectrometer (GC-ITMS) with electron and chemical ionization sources. This instrumentation will be used primarily for characterization of small biologically active molecules. Previously developed and new LC-SEI-MS/MS methods will be employed in projects including lipid peroxidation processes with a focus on trans-4-hydroxy-2-nonenal and it metabolites and the impact of prostaglandin formation in neuroinflammation. To ensure that investigators have access to the equipment and the relevant techniques and approaches, the Director will commit 100% effort, to train new users, provide regular maintenance of the instrumentation, setup and implement standard operating procedures, and provide assistance with data analysis. Currently 4 users are trained to operate independently the equipment in the MS Core facility. Because of increased need and growth of our COBRE-supported group, we will train ten additional users. The mass spectrometers coupled with LCs require regular sophisticated and costly maintenance and repairs. Thus, full service contracts that cover repairs and travel are required and have been budgeted. To enhance the use of the MS Core facility, the services of the Director will be provided and we will provide standard supplies. However, as demands for the use of the facility exceeds the level budgeted, frequent users will be required to provide their own supplies.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 在过去五年中，质谱 (MS) 核心设施为我们 COBRE 支持的团队内外的众多研究人员提供了支持。 这里的目标是继续为 UND 的研究人员提供非常高水平的设备访问和技术支持，以支持他们正在进行的研究工作。 MS 核心设施的建造和装备由我们的 COBRE I 赠款和 NCRR 提供的资金提供。该设施配备了三台色谱/质谱仪器，包括 (1) 配备纳流液相色谱的高分辨率电喷雾四极杆/飞行时间 MS (ESI-QTOF) QSTAR SL，(2) 配备纳米 LC 的 ESI 三重四极杆 MS API 3000高效液相色谱仪（LC-ESI-MS/MS），以及（3）带有离子阱质谱仪的气相色谱仪（GC-ITMS）具有电子和化学电离源。 该仪器将主要用于表征小生物活性分子。 先前开发的新 LC-SEI-MS/MS 方法将用于包括脂质过氧化过程在内的项目，重点关注反式 4-羟基-2-壬烯醛及其代谢物以及前列腺素形成对神经​​炎症的影响。 为了确保调查人员能够使用设备以及相关技术和方法，主任将投入 100% 的努力，培训新用户，提供仪器的定期维护，设置和实施标准操作程序，并提供数据分析方面的帮助。 目前，4 名用户接受了独立操作 MS Core 设施中设备的培训。 由于 COBRE 支持的团队的需求增加和发展，我们将培训另外 10 名用户。 与液相色谱相结合的质谱仪需要定期进行复杂且昂贵的维护和修理。 因此，需要涵盖维修和差旅的全面服务合同，并且已列入预算。 为了提高MS核心设施的使用，将提供主任的服务，我们将提供标准用品。 然而，由于对该设施的使用需求超出了预算水平，经常使用的用户将需要提供自己的用品。