COBRE: UND: MASS SPECTROMETRY CORE FACILITY

COBRE：UND：质谱核心设施

• 批准号: 8168377
• 负责人: Jonathan David Geiger
• 金额: $ 20.28万
• 依托单位: UNIVERSITY OF NORTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168377
• 关键词: Budgets; Centers of Research Excellence; Chemicals; Commit; Computer Retrieval of Information on Scientific Projects Database; Contract Services; Core Facility; Coupled; Data Analyses; Electrons; Ensure; Equipment; Funding; Gases; Grant; Growth; Institution; Ions; Lipid Peroxidation; Liquid substance; Maintenance; Mass Spectrum Analysis; Methods; National Center for Research Resources; Performance; Procedures; Process; Prostaglandins; Research; Research Personnel; Resolution; Resources; Services; Source; Spectrometry, Mass, Electrospray Ionization; Support Groups; Techniques; Time; Training; Travel; United States National Institutes of Health; instrument; instrumentation; ionization; mass spectrometer; microbial alkaline proteinase inhibitor; nano; neuroinflammation; repaired

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Mass Spectrometry (MS) Core facility has, for the last six years, supported numerous investigators within and outside our COBRE-supported group. The objective here is to continue providing investigators at UND with a very high level of equipment access and technical support for their ongoing research efforts. The MS Core facility was built and equipped through funding provided by our COBRE I grant and the NCRR. The facility is equipped with three chromatographic/mass spectrometric instruments including (1) a high resolution electrospray-quadrupole/time of flight MS (ESI-QTOF) QSTAR SL equipped with nano-LC, (2) a ESI-triplequadrupole MS API 3000 with high performance liquid chromatograph (LC-ESI-MS/MS), and (3) a gas chromatograph with ion trap mass spectrometer (GC-ITMS) with electron and chemical ionization sources. This instrumentation will be used primarily for characterization of small biologically active molecules. Previously developed and new LC-SEI-MS/MS methods will be employed in projects including lipid peroxidation processes with a focus on trans-4-hydroxy-2-nonenal and it metabolites and the impact of prostaglandin formation in neuroinflammation. To ensure that investigators have access to the equipment and the relevant techniques and approaches, the Director will commit 100% effort, to train new users, provide regular maintenance of the instrumentation, setup and implement standard operating procedures, and provide assistance with data analysis. Currently 4 users are trained to operate independently the equipment in the MS Core facility. Because of increased need and growth of our COBRE-supported group, we will train ten additional users. The mass spectrometers coupled with LCs require regular sophisticated and costly maintenance and repairs. Thus, full service contracts that cover repairs and travel are required and have been budgeted. To enhance the use of the MS Core facility, the services of the Director will be provided and we will provide standard supplies. However, as demands for the use of the facility exceeds the level budgeted, frequent users will be required to provide their own supplies.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 在过去的六年中，质谱（MS）核心设施支持了我们在鞋垫支撑组内外的众多研究人员。 这里的目的是继续为调查人员提供高水平的设备访问和技术支持，以供其正在进行的研究工作。 MS Core设施是通过我们的Cobre I Grant和NCRR提供的资金来建造和配备的。该设施配备了三种色谱/质谱仪器，包括（1）高分辨率电喷雾 - Quadrupole/MS飞行时间（ESI-QTOF）QSTAR SL，配备了纳米LC，（2）Esi-TriplipleQuadrupole MS API 3000带有高性能液体液体液体量表（LC-ESI-MS/MS/MS/MSS），（3），（3） （GC-ITMS）带电子和化学电离源。 该仪器将主要用于表征小的生物活性分子。 先前开发的和新的LC-SEI-MS/MS方法将用于包括脂质过氧化过程在内的项目，重点是反式4-羟基-2-非烯醇及其代谢产物以及前列腺素形成在神经膜片中的影响。 为了确保调查人员能够使用设备以及相关技术和方法，董事将付出100％的努力，培训新用户，定期维护仪器，设置和实施标准操作程序，并为数据分析提供帮助。 目前，对4名用户进行了培训，可以独立操作MS Core设施中的设备。 由于我们的玉米鞋支持组的需求增加和增长，我们将培训十个其他用户。 质谱仪与LCS结合使用，需要定期进行复杂且昂贵的维护和维修。 因此，需要涵盖维修和旅行的全面服务合同，并且已经预算了。 为了增强MS Core设施的使用，将提供主管的服务，我们将提供标准用品。 但是，由于对设施的使用需求超过了预算的水平，因此需要经常使用自己的用品。