ANALYSIS OF PROMOTER ELEMENTS IN BORRELIA BURGDORFERI

伯氏疏螺旋体启动子元件分析

• 批准号: 7562319
• 负责人: RAMESH RAMAMOORTHY
• 金额: $ 3.04万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7562319
• 关键词: Bacteria sigma factor KatF protein; Borrelia burgdorferi; Computer Retrieval of Information on Scientific Projects Database; Elements; Funding; Genes; Grant; Indium; Institution; Mutate; Mutation; Organism; OspC protein; Promoter Regions; Research; Research Personnel; Resources; Sigma Factor; Source; Specificity; United States National Institutes of Health; promoter

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In order to better understand genes regulated by RpoS, the alternative Sigma factor in the Lyme disease spirochete, we are analyzing the promoter regions of genes that are controlled by RpoS. We mutated the promoter regions of the ospC gene to identify sequence elements that confer RpoS specificity. Preliminary results indicate that the specificity of promoter recognition lies in the -10 element. We are currently confirming this by including other mutations in the ospC promoter as well as the promoter regions of at least two other genes. The results from this study will help us identify other RpoS-controlled genes in these organisms.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 为了更好地了解受 RpoS（莱姆病螺旋体中的替代 Sigma 因子）调控的基因，我们正在分析受 RpoS 控制的基因的启动子区域。 我们突变了 ospC 基因的启动子区域，以鉴定赋予 RpoS 特异性的序列元件。初步结果表明启动子识别的特异性在于-10元件。我们目前正在通过在 ospC 启动子以及至少两个其他基因的启动子区域中包含其他突变来证实这一点。这项研究的结果将帮助我们识别这些生物体中其他 RpoS 控制的基因。