GENE EXPRESSION IN BORRELIA BURGDORFERI

伯氏疏螺旋体的基因表达

• 批准号: 7562247
• 负责人: RAMESH RAMAMOORTHY
• 金额: $ 3.04万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7562247
• 关键词: Address; Antibodies; Bacteria sigma factor KatF protein; Borrelia burgdorferi; Complement; Complex; Computer Retrieval of Information on Scientific Projects Database; Event; Funding; Gene Expression; Gene Expression Regulation; Genes; Grant; HK2 gene; Human Glandular Kallikrein 2; Infection; Institution; Knock-out; OspA protein; Play; Proteins; Reagent; Regulation; Research; Research Personnel; Resources; Role; Sigma Factor; Signal Transduction Pathway; Source; Spottings; Ticks; United States National Institutes of Health; protein-histidine kinase; response; transmission process; vector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are focused on understanding regulation of gene expression in the Lyme disease spirochetes at the onset of vertebrate infection. The alternative Sigma factor, RpoS, plays a significant part during these events by regulating the expression of multiple genes including ospA and ospCthat have been shown to play important roles within the tick vector and during spirochetal transmission into the vertebrate host. The regulation of rpoS itself has been shown to be complex in B. burgdorferi and likely involves the relA/spoT gene as well as a signal transduction pathway composed of histidine kinase (Hk2)/response regulator (RRP2) and the sigma factor RpoN. We have knocked out the relA/spoT gene and are still attempting to complement the knockout with a wildtype copy of this gene. In the meantime, we are also examining the mechanism underlying the variable expression of RpoS in different strains by targeting the factors HK2, RRP2 and RpoN. We have generated an antibody against the RRP2 protein and are currently do the same for RpoN and HK2. We will use these reagents to address the issue of differential expression of RpoS.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们专注于了解莱姆病螺旋体基因表达的调控 在脊椎动物感染开始时。另一种 Sigma 因子 RpoS 通过调节包括 ospA 和 ospC 在内的多个基因的表达，在这些事件中发挥着重要作用，这些基因已被证明在蜱载体内以及螺旋体传播到脊椎动物宿主期间发挥着重要作用。在伯氏疏螺旋体中，rpoS 本身的调节非常复杂，可能涉及 relA/spoT 基因以及由组氨酸激酶 (Hk2)/反应调节器 (RRP2) 和 sigma 因子 RpoN 组成的信号转导途径。我们已经敲除 relA/spoT 基因，并且仍在尝试用该基因的野生型拷贝来补充敲除。同时，我们还通过针对HK2、RRP2和RpoN因子来研究RpoS在不同菌株中表达差异的机制。我们已经生成了针对 RRP2 蛋白的抗体，目前正在针对 RpoN 和 HK2 进行同样的操作。我们将使用这些试剂来解决 RpoS 差异表达的问题。