GENE EXPRESSION IN BORRELIA BURGDORFERI

伯氏疏螺旋体的基因表达

• 批准号: 7716187
• 负责人: RAMESH RAMAMOORTHY
• 金额: $ 2.41万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-21 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7716187
• 关键词: Bacteria sigma factor KatF protein; Borrelia burgdorferi; Complement; Complex; Computer Retrieval of Information on Scientific Projects Database; Condition; Data; Fibrinogen; Funding; Gene Expression; Gene Expression Regulation; Genes; Grant; Growth; Infection; Institution; Manuscripts; Methods; Nutrient; Organism; Pathway interactions; Regulation; Research; Research Personnel; Resources; Sigma Factor; Source; Spottings; United States National Institutes of Health; mutant

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background & Aims: Regulation of gene expression in the Lyme disease spirochetes is of fundamental importance to establishing a successful infection. The alternative Sigma factor, RpoS, is a key player that orchestrates the expression of multiple genes at the onset of infection and throughout vertebrate infection. The regulation of rpoS itself is complex and involves multiple pathways. We investigated the regulation of RpoS in this organism. Methods: We have deleted the relA/spoT gene in B. burgdorferi and subsequently complemented the relA/spoT mutant in an attempt to understand its contribution to the regulation of rpoS in this organism. Results: Preliminary results suggest that the relA/spoT gene is important for spirochetal growth under conditions of limited nutrient availability. Moreover, the expression of RpoS was also significantly higher in the wildtype and complemented strains as compared to the relA/spoT mutant. Conclusions: Our data indicate that the relA/spoT gene is one of the factors that control the expression of RpoS in this organism. This study is currently being completed and a manuscript will be prepared shortly.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 背景和目的：莱姆病螺旋体基因表达的调节对于建立成功的感染至关重要。另一种 Sigma 因子 RpoS 是在感染开始时和整个脊椎动物感染过程中协调多个基因表达的关键因素。 rpoS的调控本身很复杂，涉及多种途径。我们研究了该生物体中 RpoS 的调节。 方法：我们删除了伯氏疏螺旋体中的 relA/spoT 基因，随后补充了 relA/spoT 突变体，试图了解其对该生物体中 rpoS 调节的贡献。结果：初步结果表明，relA/spoT 基因对于营养物质可用性有限的条件下螺旋体的生长很重要。此外，与relA/spoT突变体相比，野生型和互补菌株中RpoS的表达也显着更高。结论：我们的数据表明relA/spoT基因是控制该生物体中RpoS表达的因素之一。这项研究目前正在完成，很快就会准备一份手稿。