Immunology Core

免疫学核心

• 批准号: 7667903
• 负责人: John David Altman
• 金额: $ 38.07万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7667903
• 关键词: AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Address; Adoption; Advertising; Antibodies; Arts; Biological Assay; Boxing; Categories; Cell Separation; Cells; Cellular Assay; Cellular Immunity; Charge; Chimeric Proteins; Clinical Trials; Color; Communities; Computer Analysis; Computer software; Custom; Data; Data Analyses; Development; Environment; Flow Cytometry; Grant; HIV; Hearing; Human; Immunologic Deficiency Syndromes; Immunologic Techniques; Immunology; In Vitro; Individual; International; International AIDS; Label; Laboratories; Libraries; Mediating; Mission; Monkeys; Monoclonal Antibodies; NIH Program Announcements; Pathogenesis; Performance; Peripheral Blood Mononuclear Cell; Prevention; Price; Primates; Production; Purpose; Reagent; Research; Research Personnel; Resource Sharing; Resources; Sampling; Scientist; Services; Sorting - Cell Movement; Staining method; Stains; Standardization; Training; Training and Education; Vaccinated; Validation; Virus Diseases; Visit; Work; assay development; cell mediated immune response; chemokine; cytokine; data acquisition; design; immune function; in vivo; innovation; instrument; instrumentation; member; method development; microbial; multidisciplinary; nonhuman primate; novel; pathogen; pre-clinical; repository; research study; therapeutic vaccine; vaccine development; vaccine efficacy

Under the direction of Dr. John D. Altman, the mission of Core H, the Emory CFAR Immunology Core Laboratory (ICL), is to provide the Emory community with the highest quality assessments of immunological function necessary for the study of the pathogenesis, treatment, and prevention of immunodeficiency virus infections in humans and non-human primates. The ICL will achieve this mission through the following Specific Aims: 1. Develop and perform optimized, standardized, and validated assays of cellular immune function in vaccinated subjects, in HIV-infected humans, and in SIV-infected non-human primates (NHP); 2. Create a repository of cryopreserved PBMC from naive (off-study) monkeys from the Yerkes Primate Research Center, thereby providing CFAR investigators with access to esse'ntial negative control samples for assay development and validation. 3. Achieve economies of scale through the provision of immunological reagent resources shared by multiple independent laboratories; 4. Maintain a CFAR Flow Cytometry Core, for the purpose of providing members of the Emory CFAR, and the larger AIDS research community, with the highest quality flow cytometry data and cell sorting services available, including handling of biohazardous samples; 5. Promote immunological assay education and training opportunities for CFAR investigators, the AIDS research community in Atlanta, and national and international AIDS investigators. Through these Aims, the ICL will enable all Emory CFAR investigators to take advantage of innovative and powerful modern approaches to quantitative analysis of innate and acquired humoral and cell-mediated immunity that have been developed by individual Emory investigators and scientists elsewhere. Accurate characterization of cell-mediated immune responses is essential for guiding preclinical vaccine development, for determining possible correlates of vaccine efficacy in preclinical and clinical trials, and for better understanding AIDS pathogenesis.

在约翰·D·奥特曼（John D. Altman）博士的指导下 实验室（ICL）是为了为埃默里社区提供对免疫学的最高质量评估 研究发病机理，治疗和预防免疫缺陷病毒所必需的功能 人类和非人类灵长类动物的感染。 ICL将通过以下来实现这一任务 具体目的： 1。开发和执行优化，标准化和经过验证的细胞免疫功能的测定 在感染HIV的人类和SIV感染的非人类灵长类动物（NHP）中，接种疫苗的受试者； 2。从Yerkes的Naive（研究）猴子中创建一个冷冻保存PBMC的存储库 灵长类动物研究中心，从而为CFAR调查人员提供访问权利的负面控制 用于测定开发和验证的样本。 3。通过提供免疫试剂资源共享的规模经济经济 多个独立实验室； 4。维持CFAR流式细胞仪核心，目的是为Emory CFAR的成员提供 较大的艾滋病研究界，具有最高质量的流式细胞仪数据和细胞分类 可用的服务，包括处理生物危害样品； 5。为CFAR调查人员，促进免疫学测定教育和培训机会， 亚特兰大的艾滋病研究界以及国家和国际艾滋病研究人员。 通过这些目标，ICL将使所有Emory CFAR调查人员能够利用创新和 有力的现代方法来定量分析先天和获得的体液和细胞介导的 其他地方的埃默里研究人员和科学家已经开发出的免疫力。准确的 细胞介导的免疫反应的表征对于指导临床前疫苗的发展至关重要， 用于确定临床前和临床试验中疫苗功效的可能相关性，以便更好 了解有助于发病机理。