Functional Mapping of Ethanol Avoidance in Mouse Pain

小鼠疼痛中回避乙醇的功能图谱

• 批准号: 7297847
• 负责人: Angela Renee Ozburn
• 金额: $ 2.99万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7297847
• 关键词: Alcohol consumption; Anti-Anxiety Agents; Antibodies; Behavior; Behavioral; Brain region; Cell Nucleus; Cholinergic Agents; Condition; Dose; Ethanol; Glutamates; Goals; Hybrids; Immunohistochemistry; Inbred C57BL Mice; Maps; Measures; Mining; Molecular; Mus; Neurons; Pain; Production; Property; Rewards; Self Administration; Taste Perception; Testing; alcohol behavior; alcohol effect; behavior test; cell type; cholinergic; experience; immunoreactivity; preference; research study; response

Opposing ethanol self-administration behaviors are observed when comparing two strains of mice: C57BL/6J x NZB/B1NJ F1 hybrids (B6xNZB) avoid ethanol (avoiders) after experience with high concentrations of eth- anol and C57BL/6J x FVB/NJ F1 hybrids (BGxFVB) do not (revelers). The overarching hypothesis of this proposal is that these hybrids differ in sensitivity to the aversive, but not rewarding or anxiolytic, properties of ethanol and differential levels of FosB and AFosB in specific cell types and brain regions reflects experience dependent differences in voluntary ethanol self-administration. Hybrid sensitivity to aversive, rewarding, and anxiolytic properties of ethanol will be determined by employing the following tests; conditioned taste aver- sion, conditioned place preference, modified two bottle choice, and elevated plus maze. To define neuro- circuits engaged by ethanol avoidance and revelry, FosB and AFosB immunoreactivity will be measured in ethanol experienced hybrids. Neuronal cell types of FosB and AFosB expressing neurons will also be deter- mined. Effects of strain, dose, and quantity of FosB/AFosB positive nuclei (per brain region and cell type) will be determined. These experiments will contribute to the understanding of alcohol use and abuse.

比较两种菌株时，观察到相反的乙醇自我给药行为：C57BL/6J X NZB/B1NJ F1杂种（B6XNZB）在经历了高浓度的ETH-之后避免乙醇（避免者） ANOL和C57BL/6J X FVB/NJ F1 HYBRIDS（BGXFVB）没有（狂欢者）。总体假设 建议是这些杂种在对厌恶的敏感性方面有所不同，但没有奖励或抗焦虑的特性 特定细胞类型和大脑区域中FOSB和AFOSB的乙醇和差异水平反映了经验 自愿乙醇自我管理的依赖差异。混合对厌恶，有益和的敏感性 乙醇的抗焦虑特性将通过采用以下测试来确定；条件口味平均 sion，条件的地方偏好，修改了两个瓶子的选择，并抬高了迷宫。定义神经 - 避免乙醇和狂欢，FOSB和AFOSB免疫反应性参与的电路将在 乙醇经历了杂种。表达神经元的FOSB和AFOSB的神经元细胞类型也将阻止 开采。 FOSB/AFOSB阳性核的应变，剂量和数量的影响（每个大脑区域和细胞类型）将 可以确定。这些实验将有助于理解酒精使用和滥用。