Functional Mapping of Ethanol Avoidance in Mouse Pain

小鼠疼痛中回避乙醇的功能图谱

• 批准号: 7297847
• 负责人: Angela Renee Ozburn
• 金额: $ 2.99万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7297847
• 关键词: Alcohol consumption; Anti-Anxiety Agents; Antibodies; Behavior; Behavioral; Brain region; Cell Nucleus; Cholinergic Agents; Condition; Dose; Ethanol; Glutamates; Goals; Hybrids; Immunohistochemistry; Inbred C57BL Mice; Maps; Measures; Mining; Molecular; Mus; Neurons; Pain; Production; Property; Rewards; Self Administration; Taste Perception; Testing; alcohol behavior; alcohol effect; behavior test; cell type; cholinergic; experience; immunoreactivity; preference; research study; response

Opposing ethanol self-administration behaviors are observed when comparing two strains of mice: C57BL/6J x NZB/B1NJ F1 hybrids (B6xNZB) avoid ethanol (avoiders) after experience with high concentrations of eth- anol and C57BL/6J x FVB/NJ F1 hybrids (BGxFVB) do not (revelers). The overarching hypothesis of this proposal is that these hybrids differ in sensitivity to the aversive, but not rewarding or anxiolytic, properties of ethanol and differential levels of FosB and AFosB in specific cell types and brain regions reflects experience dependent differences in voluntary ethanol self-administration. Hybrid sensitivity to aversive, rewarding, and anxiolytic properties of ethanol will be determined by employing the following tests; conditioned taste aver- sion, conditioned place preference, modified two bottle choice, and elevated plus maze. To define neuro- circuits engaged by ethanol avoidance and revelry, FosB and AFosB immunoreactivity will be measured in ethanol experienced hybrids. Neuronal cell types of FosB and AFosB expressing neurons will also be deter- mined. Effects of strain, dose, and quantity of FosB/AFosB positive nuclei (per brain region and cell type) will be determined. These experiments will contribute to the understanding of alcohol use and abuse.

比较两种小鼠品系时观察到相反的乙醇自我给药行为：C57BL/6J x NZB/B1NJ F1 杂交种 (B6xNZB) 在经历高浓度的 eth- 后避免使用乙醇（回避者） anol 和 C57BL/6J x FVB/NJ F1 杂交体 (BGxFVB) 则不会（狂欢者）。本研究的总体假设 建议是，这些混合体对厌恶属性的敏感性不同，但对奖励或抗焦虑属性的敏感性不同。 乙醇以及特定细胞类型和大脑区域中 FosB 和 AFosB 的差异水平反映了经验 自愿乙醇自我管理的依赖性差异。对厌恶、奖励和奖励的混合敏感性 乙醇的抗焦虑特性将通过以下测试来确定；条件性味觉厌恶 sion、条件性位置偏好、修改的两瓶选择和高架十字迷宫。定义神经 避免乙醇和狂欢所涉及的回路、FosB 和 AFosB 免疫反应性将在 乙醇经历了混合动力。表达 FosB 和 AFosB 的神经元细胞类型也将被阻止 开采的。 FosB/AFosB 阳性核的应变、剂量和数量（每个脑区域和细胞类型）的影响 被确定。这些实验将有助于了解酒精的使用和滥用。