Functional Mapping of Ethanol Avoidance in Mouse Pain

小鼠疼痛中回避乙醇的功能图谱

• 批准号: 7151624
• 负责人: Angela Renee Ozburn
• 金额: $ 2.99万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7151624
• 关键词: antibody; avoidance behavior; behavior test; behavioral /social science research tag; conditioning; ethanol; genetic transcription; immunofluorescence technique; laboratory mouse; molecular psychobiology; neurons; pharmacokinetics; predoctoral investigator; reinforcer; self medication; substance abuse related behavior; transcription factor

DESCRIPTION (provided by applicant): Opposing ethanol self-administration behaviors are observed when comparing two strains of mice: C57BL/6J x NZB/B1NJ F1 hybrids (B6xNZB) avoid ethanol (avoiders) after experience with high concentrations of ethanol and C57BL/6J x FVB/NJ F1 hybrids (BGxFVB) do not (revelers). The overarching hypothesis of this application is that these hybrids differ in sensitivity to the aversive, but not rewarding or anxiolytic, properties of ethanol and differential levels of FosB and deltaFosB in specific cell types and brain regions reflects experience dependent differences in voluntary ethanol self-administration. Hybrid sensitivity to aversive, rewarding, and anxiolytic properties of ethanol will be determined by employing the following tests; conditioned taste aversion, conditioned place preference, modified two bottle choice, and elevated plus maze. To define neurocircuits engaged by ethanol avoidance and revelry, FosB and deltaFosB immunoreactivity will be measured in ethanol experienced hybrids. Neuronal cell types of FosB and deltaFosB expressing neurons will also be determined. Effects of strain, dose, and quantity of FosB/deltaFosB positive nuclei (per brain region and cell type) will be determined. These experiments will contribute to the understanding of alcohol use and abuse.

描述（由申请人提供）：在比较两种小鼠品系时观察到相反的乙醇自我给药行为：C57BL/6J x NZB/B1NJ F1 杂种（B6xNZB）在经历高浓度乙醇和 C57BL/6J 后避免乙醇（回避者） x FVB/NJ F1 混合体 (BGxFVB) 则不然（狂欢者）。该应用的总体假设是，这些混合体对乙醇的厌恶性（但不是奖励性或抗焦虑性）的敏感性不同，并且特定细胞类型和大脑区域中 FosB 和 deltaFosB 的差异水平反映了自愿乙醇自我给药的经验依赖性差异。对乙醇的厌恶、奖励和抗焦虑特性的混合敏感性将通过采用以下测试来确定；条件性味觉厌恶、条件性地方偏好、改良的两瓶选择和高架十字迷宫。为了定义避免乙醇和狂欢所涉及的神经回路，将在经历过乙醇的混合体中测量 FosB 和 deltaFosB 免疫反应性。表达 FosB 和 deltaFosB 的神经元的神经元细胞类型也将被确定。将确定 FosB/deltaFosB 阳性核的应变、剂量和数量（每个脑区域和细胞类型）的影响。这些实验将有助于了解酒精的使用和滥用。