Functional Mapping of Ethanol Avoidance in Mouse Pain

小鼠疼痛中回避乙醇的功能图谱

• 批准号: 7151624
• 负责人: Angela Renee Ozburn
• 金额: $ 2.99万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7151624
• 关键词: antibody; avoidance behavior; behavior test; behavioral /social science research tag; conditioning; ethanol; genetic transcription; immunofluorescence technique; laboratory mouse; molecular psychobiology; neurons; pharmacokinetics; predoctoral investigator; reinforcer; self medication; substance abuse related behavior; transcription factor

DESCRIPTION (provided by applicant): Opposing ethanol self-administration behaviors are observed when comparing two strains of mice: C57BL/6J x NZB/B1NJ F1 hybrids (B6xNZB) avoid ethanol (avoiders) after experience with high concentrations of ethanol and C57BL/6J x FVB/NJ F1 hybrids (BGxFVB) do not (revelers). The overarching hypothesis of this application is that these hybrids differ in sensitivity to the aversive, but not rewarding or anxiolytic, properties of ethanol and differential levels of FosB and deltaFosB in specific cell types and brain regions reflects experience dependent differences in voluntary ethanol self-administration. Hybrid sensitivity to aversive, rewarding, and anxiolytic properties of ethanol will be determined by employing the following tests; conditioned taste aversion, conditioned place preference, modified two bottle choice, and elevated plus maze. To define neurocircuits engaged by ethanol avoidance and revelry, FosB and deltaFosB immunoreactivity will be measured in ethanol experienced hybrids. Neuronal cell types of FosB and deltaFosB expressing neurons will also be determined. Effects of strain, dose, and quantity of FosB/deltaFosB positive nuclei (per brain region and cell type) will be determined. These experiments will contribute to the understanding of alcohol use and abuse.

描述（由申请人提供）：比较两种菌株时观察到相反的乙醇自我给药行为：C57BL/6J X NZB/B1NJ F1 HYBRIDS（B6XNZB）避免经验丰富的乙醇和C57BL/6J x fvb/nj fvb f1 hynj（避开者）（避开者） （狂欢者）。该应用的总体假设是，这些杂种在对厌恶的敏感性方面的敏感性有所不同，但没有奖励或抗焦虑，乙醇的特性以及在特定细胞类型和大脑区域中FOSB和DeltafosB的差异水平反映了自愿性乙醇自身分配的经验差异。乙醇对厌食，有益和抗焦虑特性的杂种敏感性将通过采用以下测试来确定；条件味道厌恶，条件的地方偏好，修改了两个瓶子的选择以及抬高的迷宫。为了定义避免乙醇和狂欢的神经循环，将在乙醇经验丰富的杂种中测量FOSB和DeltafosB免疫反应性。也将确定表达神经元的FOSB和DeltafosB的神经元细胞类型。将确定FOSB/Deltafosb阳性核（每个大脑区域和细胞类型）的应变，剂量和量的影响。这些实验将有助于理解酒精使用和滥用。