A High-Throughput Crystallization Screening System for the UCSC Macromolecular X-

UCSC 大分子 X- 的高通量结晶筛选系统

• 批准号: 7390440
• 负责人: HARRY F NOLLER
• 金额: $ 25.01万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA CRUZ
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-15 至 2009-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7390440
• 关键词: Biological Sciences; Biomedical Research; California; Communities; Condition; Crystallization; Culicidae; Doctor of Philosophy; Equipment; Funding; Housing; Image; Institutes; Liquid substance; Manuals; Methods; Microscope; Operative Surgical Procedures; Range; Research; Robot; Robotics; Roentgen Rays; Screening procedure; System; Training; Wages; X-Ray Crystallography; high throughput screening; instrument; instrumentation; research study; structural biology; thymidine 5' -triphosphate

DESCRIPTION (provided by applicant): The macromolecular X-ray crystallography group at UCSC has developed a proposal to acquire automated liquid handling robotics and an imaging microscope for crystallization screening. The robot and imaging system allow for high-throughput screening of macromolecular crystallization conditions, while reducing the amount of purified material required for these experiments. The instrumentation will permit our research groups to screen ranges of crystallization conditions that would be impractical using current manual methods. Specifically, we are requesting funds to purchase a TTP Labtech mosquito. crystallization robot and CrystalPro HT-80D microscope imaging system. Both instruments are robust and offer ease of use which make them ideally suited for operation in a multi-user facility. They will be housed in space that is adjacent to the current macromolecular X-ray facility. A PhD-level facility manger will maintain the equipment and train users. We have obtained institutional support from the Division of Physical and Biological Science (PBSci) at UCSC and the California Institute for Quantitative Biomedical Research (QB3) in terms of contributing funds towards the salary of the facility manager. Furthermore, PBSci is providing space to house the equipment. The proposed instrumentation will greatly enhance the macromolecular X-ray crystallography facility at UCSC, enabling us to create a fully modern platform for carrying out structural analysis of biomolecules and to increase the accessibility of structural biology to the UCSC biomedical research community.

描述（由申请人提供）：UCSC的大分子X射线晶体学组已经开发了一项提案，以获取自动化的液体处理机器人技术和用于结晶筛选的成像显微镜。机器人和成像系统允许对大分子结晶条件进行高通量筛选，同时减少这些实验所需的纯化材料量。该仪器将允许我们的研究小组筛选出使用当前手动方法不切实际的结晶条件范围。具体来说，我们要求资金购买TTP Labtech蚊子。结晶机器人和Crystalpro HT-80D显微镜成像系统。两种仪器都有强大的功能，并提供易用性，使其非常适合在多用户设施中运行。它们将安置在与当前大分子X射线设施相邻的空间中。博士级的设施马力将维护设备和培训用户。我们已经从UCSC的物理和生物科学划分（PBSCI）和加利福尼亚定量生物医学研究所（QB3）获得了机构支持，以向设施经理的薪水捐款。此外，PBSCI正在提供容纳设备的空间。提出的仪器将大大增强UCSC的大分子X射线晶体学设施，从而使我们能够创建一个完全现代的平台，用于对生物分子进行结构性分析，并将结构生物学对UCSC生物医学研究社区的可及性提高。