Acquisition of a Cryoprobe for an 800 MHz NMR Spectrometer

获取用于 800 MHz NMR 波谱仪的冷冻探针

• 批准号: 7389812
• 负责人: STANLEY J OPELLA
• 金额: $ 23.65万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7389812
• 关键词: Acquired Immunodeficiency Syndrome; Area; Biomedical Research; Class; Communicable Diseases; Detection; Disease; Drug Design; Inflammatory; Institutes; Investigation; Lead; Malignant Neoplasms; NMR Spectroscopy; Natural Products Chemistry; Numbers; Oceanography; Pharmaceutical Chemistry; Pharmaceutical Preparations; Pharmacy Schools; Productivity; Proteins; Research; Research Personnel; Resolution; Route; Schedule; Solutions; Structure; Time; base; drug discovery; improved; instrument; instrumentation; marine natural product; novel therapeutics; protein folding; research study; structural biology

DESCRIPTION (provided by applicant): Support is requested to purchase a cryoprobe for an existing 800 MHz NMR spectrometer dedicated to solution-state NMR studies of proteins and marine natural products. By significantly improving the sensitivity of the highest field solution-state NMR spectrometer on the UCSD campus, the cryoprobe will have a broad impact on biomedical research. Not only will the requested instrumentation make new classes of experiments feasible through its combination of high sensitivity and resolution for both 1H and 13C detection, but also it will greatly enhance the overall productivity of the research groups by effectively doubling (or more) the number of experiments that can be performed on an instrument where the requests for time exceed the time available on each monthly schedule. All NIH-supported UCSD investigators whose research involves biomolecular solution-state NMR spectroscopy have contributed to this proposal. With the inclusion of researchers from the Scripps Institute of Oceanography and the new Pharmacy School, the instrumentation will enhance NIH-supported projects in two principal areas of biomedical research: structural biology and marine natural products, which provide complementary routes to new drugs for the treatment of diseases. The relevance of the instrumentation to biomedical research is in two principal areas. The Major Users are involved in structural biology, in particular studies of the structure, dynamics, and folding of proteins, and in drug discovery through the investigation of marine natural products chemistry. The combination of structure-based and medicinal-chemistry based drug design has the potential to lead to new therapeutics for diseases as diverse as cancer, AIDS and other infectious diseases, and inflammatory diseases.

描述（由申请人提供）：请求支持为现有的 800 MHz NMR 波谱仪购买冷冻探针，专用于蛋白质和海洋天然产物的溶液态 NMR 研究。通过显着提高加州大学圣地亚哥分校校园最高场溶液态核磁共振波谱仪的灵敏度，冷冻探针将对生物医学研究产生广泛的影响。所需的仪器不仅将通过结合 1H 和 13C 检测的高灵敏度和分辨率使新型实验变得可行，而且还将通过有效地使研究小组的数量增加一倍（或更多）来大大提高研究小组的整体生产力。当时间请求超出每个月计划的可用时间时，可以在仪器上执行实验。所有 NIH 支持的 UCSD 研究人员的研究涉及生物分子溶液态核磁共振波谱，都为这项提案做出了贡献。随着斯克里普斯海洋研究所和新药学院的研究人员的加入，该仪器将增强美国国立卫生研究院支持的生物医学研究两个主要领域的项目：结构生物学和海洋天然产物，为治疗新药提供补充途径的疾病。仪器与生物医学研究的相关性有两个主要领域。主要用户涉及结构生物学，特别是蛋白质的结构、动力学和折叠的研究，以及通过研究海洋天然产物化学来发现药物。基于结构和基于药物化学的药物设计相结合，有可能为癌症、艾滋病和其他传染病以及炎症性疾病等多种疾病带来新的治疗方法。