Core B: Animal and Analytical Core

核心 B：动物和分析核心

基本信息

批准号：
7285834
负责人：
BAOLI YANG
金额：
$ 23.85万
依托单位：
UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-03-01 至 2012-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7285834
关键词：
Alleles Animal Model Animals Breeding Caring Cells Collaborations Core Facility Creatinine Cultured Cells Feline Immunodeficiency Virus Funding Gene Targeting Generations Genes Genome Genotype Grant Infection Institution Iowa Knock-in Mouse Knock-out Knockout Mice Laboratories LacZ Genes Medicine Methods Microinjections Modeling Mus Mutant Strains Mice Phosphorous Point Mutation Process Purpose Quality Control RNA Interference Research Personnel Resources Serum Standards of Weights and Measures Support of Research Techniques Technology Tissue Extracts Tissues Transfection Transgenic Animals Transgenic Model Transgenic Organisms Universities Urine Yang animal facility base college embryonic stem cell experience gene transfer vector immortalized cell instrumentation knockout animal mouse model mutant programs small hairpin RNA transmission process vector

项目摘要

The purpose of this Mouse, Analytical and Cell Culture Core is to provide centralized care, breeding, genotyping and quality control of transgenic and knockout animals for use by investigators in the program project. In addition, the core will generate new transgenic and gene targeted mouse models where needed by program investigators. The core will also provide a mechanism for the distribution of new experimental transgenic and knockout models to either depository (such as the Jackson Laboratory Induced Mutant Resource) or to other investigators at the University of Iowa and outside the institution. Furthermore, the core will also provide centralized analyses of serum, urine and tissue extract for Na+, K+, CI-, Mg2+, Ca2+, phosphorous and creatinine. Additionally, it will generate immortalized cells and provide cells and tissues to each of the investigators of this Program Project. The overall Core is directed by Dr. Baoli Yang, who currently serves as Director of the Gene Targeting Core Facility on the campus at the University of Iowa.

该小鼠、分析和细胞培养核心的目的是提供集中护理、育种、供项目研究人员使用的转基因和基因敲除动物的基因分型和质量控制项目。此外，核心将在需要时生成新的转基因和基因靶向小鼠模型由计划调查员。核心还将提供一种分配新实验的机制任一保藏库的转基因和敲除模型（例如杰克逊实验室诱导突变体资源）或爱荷华大学内外的其他研究人员。此外，核心还将提供血清、尿液和组织提取物中 Na+、K+、CI-、Mg2+、Ca2+ 的集中分析，磷和肌酐。此外，它将产生永生化细胞并提供细胞和组织该计划项目的每位研究者。总体核心由杨宝利博士指导，目前担任爱荷华大学校园基因靶向核心设施的主任。