Cell Imaging, Analysis and Microinjection

细胞成像、分析和显微注射

• 批准号: 7425545
• 负责人: JAMES R FERAMISCO
• 金额: $ 6.18万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-12-01 至 2012-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7425545
• 关键词: 3-Dimensional; Area; Cells; Computers; Data Set; Digital Imaging Shared Resource; Frequencies; Funding; Hand; Health Services Accessibility; Image Analysis; Imagery; Individual; Laboratories; Microinjections; Microscope; Microscopy; Online Systems; Research; Research Personnel; Resources; Scientist; Services; Somatic Cell; System; Time; Training; Visualization software; cellular imaging; computerized data processing; member; programs; supercomputer; tool

The Cell Imaging, Analysis, & Microinjection Core (CIAMC) comprises valuable, modern tools that are made available as routine services to Program Project members. The services provided have been crucial for individual laboratory projects, and intra-programmatic research. The CIAMC was established in the original application to meet the growing needs of the investigators in this area. The CIAMC has evolved to now take advantage of microscopy resources in the Moores UCSD Digital Imaging Shared Resource (DISR). We propose in this application to fund a part-time research assistant to provide dedicated support to PPG members by 1) providing standardized training in the use of these 3-D fluorescence.microscopes and the somatic cell microinjection system, 2) training individuals within this PPG to take full advantage of the enhanced scientific visualization software available in our group, and 3) providing hands-on help with the use of these microscopes. A unique feature of our facility is its partnership with computer scientists at the VisLab in the San Diego Supercomputer Center (SDSC). They have created new tools for enhanced 3-D visualization of cells, web-based data processing, and have made use of the virtually unlimited computational power of the SDSC to facilitate storage and analysis of very large data sets (http://vis.sdsc.edu/research/cancer.html). With increasing frequency, current PPG investigators are making use of these enhanced features, and the proposed CIAMC will facilitate the ability of PPG members to access these services in a timely manner.

细胞成像，分析和显微注射核心（CIAMC）包括有价值的现代工具 作为计划成员的常规服务。提供的服务对于 个别实验室项目和概括性研究。 CIAMC是在原始的 满足该领域调查人员不断增长的需求的申请。 CIAMC已经演变为现在 Moores UCSD数字成像共享资源（DISR）中显微镜资源的优势。我们 在此申请中提议为兼职研究助理提供资金，以向PPG提供专门的支持 成员1）在使用这些3-D荧光时提供标准化的培训。 体细胞微注射系统，2）在此PPG中培训个体以充分利用 增强的科学可视化软件以及3）提供动手帮助方面的帮助 这些显微镜。我们设施的一个独特功能是它与Vislab的计算机科学家的合作伙伴关系 在圣地亚哥超级计算机中心（SDSC）中。他们创建了增强3-D的新工具 单元格的可视化，基于Web的数据处理，并使用了几乎无限的 SDSC的计算能力促进了非常大的数据集的存储和分析 （http://vis.sdsc.edu/research/cancer.html）。随着频率的增加，当前的PPG研究人员正在制造 使用这些增强功能，拟议的CIAMC将有助于PPG成员的能力 及时访问这些服务。