Digital Imaging Core

数字成像核心

• 批准号: 7928762
• 负责人: JAMES R FERAMISCO
• 金额: $ 35.81万
• 依托单位: VETERANS MEDICAL RESEARCH FDN/SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7928762
• 关键词: 3-Dimensional; Address; Animals; Cardiac; Cell physiology; Cells; Color; Fluorescence Microscopy; Gene Expression; Gene Transfer; Genes; Goals; Image; Image Analysis; Imagery; Imaging Techniques; Immunofluorescence Immunologic; Individual; Inflammatory Response; Life; Location; Microscope; Microscopy; Modems; Molecular; Organism; Participant; Pathology; Reagent; Research; Research Personnel; Research Project Grants; Resolution; Resources; Sampling; Services; Staining method; Stains; System; Time; Tissues; Validation; animal tissue; base; cell type; cellular imaging; cellular transduction; cost; digital imaging; multi-photon; programs; supercomputer; tool; transgene expression

The Digital Imaging Core (Core A) comprises valuable and modem tools, which are to be made available as routine services to program participants. A major goal of Core A is to provide uniform services to enable Program investigators to validate transduced cardiac cells and tissues. Validation includes assessing level of gene expression, cell-to-cell variability in expression, and structural or functional consequences to transduced cells of the altered gene expression. Services include 3-dimensional cell and tissue digital imaging with multi-color fluorescence microscopy for both living and fixed samples. Since the last review, Core A has evolved to include access to a multi-photon and a multi-spectral confocal microscope fitted for live-cell imaging, and an intra-vital confocal system for live animal imaging. Many components of this Program involve analysis of gene expression in cells and tissues, and as such this Core will be widely utilized. Based upon high resolution imaging techniques, including a DeltaVision deconvolution microscope system and UNIX workstations, these services include quantitative image analysis, time-lapse microscopy and volume 3-dimensional renderings of cells and tissues. Through Core A image analysis is greatly enhanced through interactions with the VisLab at the San Diego Supercomputer Center, with use of NPACI Scalable Visualization Tools. These tools allow for real-time exploration of the special locations of 3 or 4 cellular components in 3-D. These services have been crucial for individual lab projects and intra-programmatic research projects in this new program. In this service, animal tissues following transgene expression can be analyzed at several levels, including gross pathology and 3- dimensional tissue imaging with immunofluorescence staining. The service will be able to provide imaging analysis of various aspects of the targeted tissues, including specific cell type identification of transgene expression, tissue inflammatory responses, resultant tissue organization, and any changes in cellular gene expression or sarcomeric structural organization. Cells transduced in culture can be imaged as live cells revealing temporal relationships or as fixed samples, stained with immunofluorescent reagents for subsequent 3- dimensional structural analysis. As research goals and efforts within our Program address the molecular and cellular functions of endogenous and transduced genes, this resource promises to provide cost-effective support for these cellular, tissue and animal-based studies.

数字成像核心（核心A）包括有价值的调制解调器工具，可以作为 计划参与者的常规服务。核心A的主要目标是提供统一的服务以启用程序 研究人员验证转导的心脏细胞和组织。验证包括评估基因水平 表达，表达中的细胞对细胞变异性以及对转导细胞的结构或功能后果 基因表达改变。服务包括具有多色的3维电池和组织数字成像 生存和固定样品的荧光显微镜。自上次评论以来，核心A已演变为包括 访问适用于活细胞成像的多光子和多光谱共聚焦显微镜以及重要的 活动物成像的共聚焦系统。该程序的许多组成部分涉及对基因表达的分析 细胞和组织，因此将广泛使用这种核心。基于高分辨率成像技术， 包括Deltavision Deonvolution显微镜系统和UNIX工作站，这些服务包括 定量图像分析，延时显微镜以及细胞和组织的体积3维渲染。 通过核心，图像分析通过与圣地亚哥的Vislab的互动大大增强 超级计算机中心，使用NPACI可伸缩可视化工具。这些工具可以实时 3-D中3或4个细胞成分的特殊位置的探索。这些服务至关重要 这个新计划中的单个实验室项目和典型的研究项目。在这项服务中，动物 转基因表达后的组织可以在多个级别进行分析，包括总病理和3-- 尺寸组织成像，并具有免疫荧光染色。该服务将能够提供成像 分析目标组织的各个方面，包括特定的细胞类型鉴定 表达，组织炎症反应，结果组织组织以及细胞基因的任何变化 表达或肉类结构组织。在培养中转导的细胞可以成像为活细胞揭示 时间关系或作为固定样品，用免疫荧光试剂染色，以进行3-- 维度结构分析。随着我们计划中的研究目标和努力解决分子和 内源性和转导基因的细胞功能，该资源有望提供具有成本效益的支持 对于这些细胞，组织和基于动物的研究。