CORE--DIGITAL IMAGING AND CELL MICROINJECTION

核心——数字成像和细胞显微注射

• 批准号: 6599261
• 负责人: JAMES R FERAMISCO
• 金额: $ 18.37万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-11 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6599261
• 关键词: biomedical facility; digital imaging; microinjections

The Digital Imaging and Cell Microinjection (DICM) Shared Resource comprises valuable and modern tools which are made available as routine services to Cancer Center Members. Services include high-resolution digital deconvolution microscopy, digital epi-fluorescence and brightfield microscopy, quantitative image analysis, cell microinjection and subsequent biological analyses, photo-production and training and advice. These services have been crucial for individual lab projects, intra- programmatic research and inter-programmatic research. As research goals and efforts within our Center which address our molecular and cellular functions and intracellular localization of genes implicated in the genesis or growth modulation of cancer cells are emerging with increasing frequency, the Center has taken steps to provide cost-effective support for these cellular and tissue-based studies. This evolution stems from the fact that investigators, such as those in our Cancer Biology and clinical investigation. Programs, have been identifying candidate candidate genes which may be responsible. This has reinforced the fact that the highly important task of solving the functions of these genes is often rate limiting, which is an essential feature of the above programs as well as others in our Center. As clues to the functions of candidate genes are often gained by studying the location of the gene products in cells, the DICM Shared Resource meets a portion of these needs. According, the Resource has been used by over 30 laboratories within the Cancer Center.

数字成像和细胞显微注射（DICM）共享资源包括有价值的现代工具，这些工具可作为癌症中心成员的常规服务提供。服务包括高分辨率的数字反卷积显微镜，数字荧光和Brightfield显微镜，定量图像分析，细胞显微注射以及随后的生物学分析，照片产生以及培训以及培训和建议。这些服务对于单个实验室项目，程序内研究和程序间研究至关重要。随着我们中心内的研究目标和努力解决我们的分子和细胞功能以及与癌细胞的发生或生长调节有关的基因的细胞内定位，随着频率的增加而出现，该中心已采取步骤为这些基于细胞和组织的研究提供成本效益的支持。这种进化源于以下事实：研究人员，例如我们的癌症生物学和临床研究中的研究者。程序，一直在确定可能负责的候选候选基因。这加强了这样一个事实，即解决这些基因功能的非常重要的任务通常是限制速率，这是上述程序以及我们中心中其他程序的重要特征。由于通常通过研究基因产物在细胞中的位置来获得候选基因功能的线索，DICM共享资源满足了这些需求的一部分。根据癌症中心内30多个实验室的资源，该资源已使用。