Improving the Detection Limit of Potentiometric Sensors

提高电位传感器的检测限

• 批准号: 7495062
• 负责人: JOSEPH WANG
• 金额: $ 27.16万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-25 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7495062
• 关键词: Binding; Biosensing Techniques; Biosensor; Calibration; Confined Spaces; Coupled; Coupling; DNA; Detection; Development; Devices; Drug Monitoring; Electrolytes; Event; Grant; Ions; Knowledge; Label; Laws; Liquid substance; Measurable; Measures; Membrane; Metals; Methodology; Methods; Polymers; Potentiometry; Process; Publications; Sampling; Surface; Techniques; Transducers; base; concept; controlled release; design; improved; nanoparticle; novel; novel strategies; oxidation; sensor

DESCRIPTION (provided by applicant): The discovery that the detection limit of potentiometric sensors may be improved by 3 to 6 orders of magnitude of traditionally accepted levels have drastically changed the field of ion sensing. In this continuation proposal, new avenues will be explored to more completely assess the limits of such sensors and to design novel sensing concepts based on ion fluxes at ion-selective membranes. Since potentiometry, as one-dimensional technique, is not subject to scaling laws, the limits of potentiometric sensors will be explored in confined sample volumes. This knowledge will be used to design ultra-sensitive DNA detection devices based on metal nanoparticle probes that would be potentiometrically detected after oxidation. Potentiometry will be coupled to analyte enrichment processes, in analogy to their voltammetric counterparts for a further drastic decrease in detection limit that may surpass that of any other electrochemical method. Ion fluxes at ion-selective membranes, induced chemically and galvanostatically, will be used to design 10-fold more sensitive measuring devices than in traditional potentiometry, which will be very useful for electrolyte and drug monitoring. The perturbance of such fluxes by surface binding events will be used to explore novel approaches to biosensing at liquid-polymer interfaces.

描述（由申请人提供）：电位传感器的检测限可以比传统接受的水平提高 3 至 6 个数量级的发现已经极大地改变了离子感测领域。在这个延续提案中，将探索新的途径，以更全面地评估此类传感器的局限性，并根据离子选择性膜上的离子通量设计新颖的传感概念。由于电位测量作为一维技术，不受比例定律的约束，因此将在有限的样品体积中探索电位传感器的局限性。这些知识将用于设计基于金属纳米粒子探针的超灵敏 DNA 检测装置，这些探针将在氧化后进行电位检测。电位测定法将与分析物富集过程相结合，类似于伏安法，可进一步大幅降低检测限，可能超过任何其他电化学方法。通过化学和恒电流诱导的离子选择性膜上的离子通量将用于设计比传统电位测定法灵敏 10 倍的测量装置，这对于电解质和药物监测非常有用。表面结合事件对此类通量的扰动将用于探索液体-聚合物界面生物传感的新方法。

项目成果

Sensitivity and working range of backside calibration potentiometry.

背面校准电位的灵敏度和工作范围。

• DOI: 10.1021/ac071248a
• 发表时间: 2007
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Ngeontae,Wittaya;Xu,Yida;Xu,Chao;Aeungmaitrepirom,Wanlapa;Tuntulani,Thawatchai;Pretsch,Ernö;Bakker,Eric
• 通讯作者: Bakker,Eric

Comparison Between Potentiometric and Stripping Voltammetric Detection of Trace Metals: Measurements of Cadmium and Lead in the Presence of Thalium, Indium, and Tin.

微量金属的电位法和溶出伏安法检测的比较：铊、铟和锡存在下镉和铅的测量。

• DOI: 10.1002/elan.200904613
• 发表时间: 2009
• 期刊: Electroanalysis
• 影响因子: 3
• 作者: Chumbimuni-Torres,KarinY;Calvo-Marzal,Percy;Wang,Joseph
• 通讯作者: Wang,Joseph

Prussian Blue Dispersed Sphere Catalytic Labels for Amplified Electronic Detection of DNA.

• DOI: 10.1002/elan.200704063
• 发表时间: 2008-02
• 期刊: Electroanalysis
• 影响因子: 3
• 作者: Siriwan Suwansa-ard;Yun Xiang;R. Bash;P. Thavarungkul;P. Kanatharana;Joseph Wang

High-temperature potentiometry: modulated response of ion-selective electrodes during heat pulses.

• DOI: 10.1021/ac902191h
• 发表时间: 2009-12-15
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: Chumbimuni-Torres, Karin Y.;Thammakhet, Chongdee;Galik, Michal;Calvo-Marzal, Percy;Wu, Jie;Bakker, Eric;Flechsig, Gerd-Uwe;Wang, Joseph
• 通讯作者: Wang, Joseph

Limitations of current polarization for lowering the detection limit of potentiometric polymeric membrane sensors.

• DOI: 10.1021/ac802588j
• 发表时间: 2009-05-01
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: Hoefler, Lajos;Bedlechowicz, Iwona;Vigassy, Tamas;Gyurcsanyi, Robert E.;Bakker, Eric;Pretsch, Ernoe
• 通讯作者: Pretsch, Ernoe