DNA BIOSENSOR ARRAY FOR SINGLE CELL GENOMICS ANALYSIS

用于单细胞基因组分析的 DNA 生物传感器阵列

• 批准号: 6039889
• 负责人: JOSEPH WANG
• 金额: $ 19.14万
• 依托单位: NEW MEXICO STATE UNIVERSITY LAS CRUCES
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-28 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6039889
• 关键词: DNA; bioengineering /biomedical engineering; biomedical equipment development; biosensor device; electrochemistry; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; single cell analysis

The goal of this collaborative project is to develop an automated hand-held micromachined electrochemical flow analyzer for high-speed single-cell genomics analysis. Innovative biosensor technology, based on new highly selective and sensitive PNA-dendrimer recognition chemistry, high-density parallel-channel hybridization microreactors, a label-free electrochemical detection of the released targets at downstream microelectrodes, and state-of-the-art microfabrication/micromachining technology will be used for meeting the challenges of rapid and sensitive single-cell nucleic-acid sequencing.

该合作项目的目标是开发一种自动化手持式微机械电化学流分析仪，用于高速单细胞基因组学分析。 创新的生物传感器技术，基于新型高选择性和灵敏的 PNA 树枝状大分子识别化学、高密度平行通道杂交微反应器、下游微电极释放靶标的无标记电化学检测以及最先进的微加工/微加工技术将用于应对快速、灵敏的单细胞核酸测序的挑战。