A Rapid On-Site Human Fecal Contamination Biosensor

快速现场人类粪便污染生物传感器

• 批准号: 6879294
• 负责人: JACK R. U'REN
• 金额: $ 8.01万
• 依托单位: SAIGENE CORPORATION
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2006-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6879294
• 关键词: Bacteroidaceae; Bacteroides; animal tissue; bacterial DNA; bacterial RNA; bioengineering /biomedical engineering; biohazard detection; biomedical equipment development; biosensor device; biotechnology; bioterrorism /chemical warfare; environmental contamination; feces analysis; fiber optics; food sanitation; human tissue; nucleic acid hybridization; oligonucleotides; portable biomedical equipment; water sampling /testing

DESCRIPTION (provided by applicant): Human fecal contamination represents a serious health hazard for the transmission of waterborne diseases such as typhoid fever, cholera, and hepatitis A. Therefore, sensitive measurements of water supplies and food handling processes for fecal microorganism are of great value in identifying dangerous human fecal contamination and tracking its source. The development of such a rapid, portable, human fecal contamination biosensor is the overall goal of this program. The Specific Aims of this six-month Phase I program are: 1) Determine the sensitivity, specificity, and reuse of an optical fiber based evanescent wave DNA hybridization assay using synthetic oligos, 2) Determine the sensitivity and specificity of the optical fiber hybridization assay targeting Bacteroides and Prevotella rRNA sequences using human and animal fecal samples, and 3) Determine the stability and reproducibility of the assay components for long-term use.

描述（由申请人提供）： 人类粪便污染代表了对水传播疾病（例如伤寒，霍乱和肝炎）的严重健康危害。因此，对粪便微生物的敏感测量和食品处理过程的敏感测量在识别危险的危险人类粪便污染和跟踪其来源方面具有很大的价值。这种计划的总体目标是这种快速，便携式，人类粪便污染生物传感器的发展。 The Specific Aims of this six-month Phase I program are: 1) Determine the sensitivity, specificity, and reuse of an optical fiber based evanescent wave DNA hybridization assay using synthetic oligos, 2) Determine the sensitivity and specificity of the optical fiber hybridization assay targeting Bacteroides and Prevotella rRNA sequences using human and animal fecal samples, and 3) Determine the stability and reproducibility of the测定长期使用的组件。