Bacterial Pathogen Amplification & Real-Time Detection

细菌病原体扩增

• 批准号: 6831824
• 负责人: JACK R. U'REN
• 金额: $ 36.2万
• 依托单位: SAIGENE CORPORATION
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6831824
• 关键词: Campylobacter; Escherichia coli; Listeria; Salmonella; Shigella; Vibrio; Yersinia; bacterial food poisoning; biohazard detection; biotechnology; bioterrorism /chemical warfare; food processing /preparation; growth media; microorganism culture; nucleic acid probes; nucleic acid purification; nucleic acid quantitation /detection; polymerase chain reaction; technology /technique development; water sampling /testing

DESCRIPTION (provided by applicant): As we all know, bio-terrorism in America is a reality. However in addition to the Category A agents like anthrax, Yersinia pestis and smallpox, which are difficult to safely grow and disseminate, exist the Category B agents that could be used to infect our food or water supply. These organisms include bacterial pathogens, protozoa, and viruses. In addition to these natural pathogenic organisms they could also be genetically engineered to increase their virulence or resist standard antibiotic treatments. Therefore new methods for rapid food and waterborne pathogen detection is greatly needed especially if it can also be used to identify drug sensitivity within these organisms. This program will develop such tests. In addition to bio-terrorism concerns, food-borne diseases cause 76-million illnesses, including 325,000 hospitalizations and 5,000 deaths in the US each year. More than 200 known diseases are transmitted through food and drink. Traditional detection of food-borne pathogens has relied on microbiologic techniques. These standard culture methods recommended by the AOAC require between 5 to 7 days to complete. Meanwhile, the product is held for shipment, increasing storage costs and reducing the product shelf life. In the case of our water supply, Public Health Service warnings are released when problems are identified, usually after the product is already released for public consumption. Consequently, rapid methods for food and water pathogen detection are greatly needed to protect the public health. The overall goal of this program is to develop an integrated isothermal DNA amplification and detection system capable of rapidly identifying a variety of food and waterborne pathogens. All of the NIAID Biodefense Category B food and waterborne bacterial pathogens will be detected in this program. A single integrated system capable of isothermal amplifying and detecting all of these organisms in a closed device is proposed.

描述（由申请人提供）：众所周知，美国的生物恐怖主义是现实。然而，除了A类A类药物之外，诸如炭疽病，耶尔森氏菌和天花很难安全地生长和传播，还存在可用于感染我们的食物或供水的B类药物。这些生物包括细菌病原体，原生动物和病毒。除了这些天然的致病生物外，它们也可以在基因上进行基因设计，以增加其毒力或抵抗标准的抗生素治疗。因此，非常需要新的快速食品和水传播病原体检测的方法，尤其是在这些生物体内还可以使用药物敏感性的情况下。该程序将开发此类测试。 除了生物恐怖主义问题外，食物传播的疾病每年造成7600万疾病，包括325,000例住院和5,000例死亡。通过食物和饮料传播了200多种已知疾病。传统的食物传播病原体检测依赖于微生物技术。 AOAC推荐的这些标准培养方法需要5到7天才能完成。 同时，该产品用于发货，增加存储成本并缩短产品保质期。就我们的供水而言，通常在产品已发布以供公众消费之后，在发现问题时会发布公共卫生服务警告。因此，需要快速的食物和水病原体检测方法来保护公共卫生。 该程序的总体目标是开发一个能够快速识别各种食物和水传播病原体的集成等温DNA扩增和检测系统。该计划将检测到所有NIAID生物浮雕类别B类食物和水性细菌病原体。提出了一个能够在封闭设备中放大并检测所有这些生物的单个集成系统。