Portable Optical Imager for Multiplexed Foodborne Pathogen Detection

用于多重食源性病原体检测的便携式光学成像仪

• 批准号: 10484520
• 负责人: Zhongjian Hu
• 金额: $ 22.5万
• 依托单位: NANOHMICS, INC.
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10484520
• 关键词: Affinity; Antibodies; Area; Bacteria; Binding; Biological Assay; Biosensing Techniques; Biosensor; Collection; Consumption; Custom; Deposition; Detection; Development; Devices; Disease Outbreaks; Early Diagnosis; Electromagnetics; Engineering; Enterovirus; Enzyme-Linked Immunosorbent Assay; Epidemic; Escherichia coli O157; Feedback; Food; Food Contamination; Food Supply; Generations; Glass; Goals; Hour; Image; Immobilization; Infrastructure; Label; Laboratories; Lasers; Light; Liquid substance; Measurement; Measures; Medical; Methods; Microfabrication; Miniaturization; Monitor; Noise; Optics; Pathogen detection; Performance; Phase; Polymerase Chain Reaction; Positioning Attribute; Public Health; Rapid diagnostics; Research; Robotics; Sampling; Series; Source; Spectrum Analysis; Spottings; Staphylococcus aureus; Structure; Sucrose; Surface; Surface Properties; Swab; Techniques; Technology; Temperature; Testing; Time; Training; Uncertainty; United States Department of Agriculture; Variant; Viral; Virus; Work; aptamer; aqueous; base; clinical diagnostics; cost; cost effective; density; design; detection limit; detector; diagnostic technologies; diagnostic tool; evaluation/testing; foodborne illness; foodborne pathogen; innovation; instrumentation; lens; miniaturize; multiplex detection; optical imager; optical sensor; pathogen; plasmonics; point-of-care diagnostics; portability; prototype; rapid detection; sensor; simulation; transmission process

Portable Optical Imager for Multiplexed Foodborne Pathogen Detection Project Summary Food contamination caused by pathogens is a significant public health concern for consumers worldwide. Critical aspects of limiting foodborne disease outbreaks and epidemic involve early detection and rapid diagnostics of foodborne pathogens which require sensitive, multiplexed devices that enable point-of-care (POC) diagnostics. New generations of rapid (detection in minutes to hours), portable, multiplexed and sensitive biosensors can significantly aid in delivery of a safer food supply. Nanohmics proposes to develop a low-cost portable optical imager that is capable of fast (< 30 minutes), sensitive (tens to hundreds of bacteria or viruses), and multiplexed testing of up to tens of foodborne pathogens from fresh produce swab fluids or rinsates and comparable food surface samples. Specifically, the proposed research will integrate low-cost large-area pixelated nanohole array (NHA) biosensing chips and economical optical and electronical components in to a compact, highly portable, battery-operated and rugged biosensor device. While NHA plasmonic biosensors already exist as bench-level laboratory experimental set ups, the technology has not been innovated to make it portable and multiplexed for field use to test numerous imported or domestic foods onsite where the FDA and USDA are overwhelmed with samples. Compared to conventional reflection-type plasmonic biosensors employing a prism, NHAs support collinear excitation and collection configuration. This not only significantly simplifies alignment and but also enables small footprint and highly multiplexed biosensing. Nanohmics will microspot NHA-based pixel arrays with different bioreceptors where the type of target is encoded in the position of the corresponding spot pixels. The team will engineer and construct an optical imager using a wide-beam red laser module, a miniaturized imaging lens, and a CMOS detector, and integrate the bioreceptor-pixelated NHA biosensing chips with the optical imager for field-portable rapid multiplexed testing of infectious pathogens. The overall integrated device is envisioned to be compact (~ 5 × 5 × 10 cm3), light (~ 50 g), and low-cost (~ $ 400 including fabrication and parts). Nanohmics aims at rapid (< 30 minutes) and sensitive detection with limit of detection in the tens to low hundreds of bacteria and viruses per mL.

用于多路复用食物病原体检测的便携式光学成像仪 项目摘要 病原体引起的粮食污染是全球消费者的重要公共卫生问题。 限制食源性疾病暴发和流行病的关键方面涉及早期发现和快速 需要敏感的多重设备的食源性病原体的诊断，以启用应答点 （POC）诊断。新一代的快速（以几分钟至几小时的范围），便携，多路复用和 敏感的生物传感器可以大大帮助提供安全的食物供应。 Nanohmics提案，以开发一种能够快速的低成本便携式光学成像仪（<30） 分钟），敏感（数十个至数百种细菌或病毒），以及多达数十个的多路复用测试 新鲜农产品拭子液体或冲洗液和可比食物表面的食物生存的病原体 样本。特别是，拟议的研究将整合低成本的大区块纳米霍尔 阵列（NHA）生物传感芯片以及经济的光学和电子组件， 高度便携，电池操作和坚固的生物传感器设备。而NHA塑料生物传感器 作为基准级实验室实验设置已经存在，该技术并没有创新 它可移植和多重用于现场，以测试FDA的众多进口或家用食品 USDA不知所措。与常规反射型浆膜生物传感器相比 使用棱镜，NHAS支持共线兴奋和收集配置。这不仅是 显着简化了对齐，但也可以实现小的足迹和高度多重的生物传感。 Nanohmics将带有Microspot NHA基于NHA的像素阵列，其目标类型为 在相应的点像素的位置编码。团队将设计并构建光学 成像仪使用宽光束红色激光模块，微型成像透镜和CMOS检测器，以及 与光学成像仪相结合的生物感受器像素化的NHA生物传感芯片以进行现场 - 可容纳快速快速 传染病的多重测试。设想整体集成设备是紧凑的（〜5 ×5×10 cm3），光（〜50 g）和低成本（包括制造和零件）。 Nanohmics的目标 快速（<30分钟）和敏感检测，在数十个低到低的细菌中检测限 和每毫升病毒。