STRUCTURAL STUDIES OF THE CLASS I LIGASE RIBOZYME

I 类连接酶核酶的结构研究

• 批准号: 7369507
• 负责人: DAVID P BARTEL
• 金额: $ 0.13万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7369507
• 关键词: STRUCTURAL; STUDIES; CLASS; LIGASE; RIBOZYME

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The class I ligase ribozyme was generated de novo from random sequences. This catalytic RNA accelerates the attack of a small oligonucleotide's 3'OH on its own 5'-terminal triphosphate, yielding a 3'-5' phosphodiester linkage and pyrophosphate. We have generated variants of the ribozyme which are perform primer extension to produced a general polymerase ribozyme which can extend any template-primer pair, often by up to 14 nucleotides. In the RNA world hypothesis, this type of RNA-dependent RNA-polymerase activity would have been a critical component of the processes that led to the origins of life. Thus the class I ligase ribozyme is an extremely attractive target for crystallographic analysis in order to understand the structural basis for this primordial enzymology.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。 I类连接酶核酶是由随机序列从头开始产生的。该催化RNA加速了小型寡核苷酸的3'OH在其自己的5'-末端三磷酸末端的攻击，从而产生3'-5'-5'磷酸二酯链接和焦磷酸。我们已经产生了核酶的变体，这些变体是执行底漆扩展的，从而产生了一般的聚合酶核酶，该聚合酶核酶可以扩展任何模板 - 蛋白酶对，通常是14个核苷酸。在RNA世界假设中，这种类型的RNA依赖性RNA聚合酶活性将是导致生命起源的过程的关键组成部分。因此，I类连接酶核酶是晶体学分析的极具吸引力的靶标，以了解这种原始酶学的结构基础。