CRYSTAL STRUCTURE OF THE CATALYTIC CORE OF AN RNA POLYMERASE RIBOZYME

RNA聚合酶核酶催化核心的晶体结构

• 批准号: 7955090
• 负责人: DAVID P BARTEL
• 金额: $ 0.51万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955090
• 关键词: Catalytic Domain; Catalytic RNA; Chemistry; Computer Retrieval of Information on Scientific Projects Database; DNA-Directed RNA Polymerase; Funding; Grant; Institution; Ligase; Minor; Oligonucleotides; RNA; Research; Research Personnel; Resources; Source; Structure; United States National Institutes of Health; Variant; base; nucleoside triphosphate; phosphodiester; polymerization; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The class I ligase ribozyme promotes the formation of a 3'-5' phosphodiester linkage between a small oligonucleotide substrate and its own 5' terminus, requiring both the nucleophile and electrophile to be situated within the context of a Watson-Crick pair. This chemistry  among the fastest yet observed for any catalytic RNA  is equivalent to a single turnover of template-directed RNA polymerization, an activity that would have been requisite in the RNA World. Indeed, minor variants of the ligase are capable of catalyzing high-fidelity template-directed RNA polymerization using exogenous nucleoside triphosphates, and the ligase constitutes the catalytic core for a general RNA polymerase ribozyme. To understand the basis of RNA-catalyzed RNA-dependent RNA polymerization, we have set out to solve the crystal structure of the ligase.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 I类连接酶核酶促进了小寡核苷酸底物与其自己的5'末端之间的3'-5'磷酸二酯连接形成，要求在沃森 - 克里克对的背景下，需要亲核和电力剂。 对于任何催化RNA，这种化学反应之间的化学性质等同于模板定向的RNA聚合化的单个营业额，这是RNA世界中必不可少的活性。 实际上，连接酶的次要变体能够使用外源性核苷三磷酸化催化高保真模板指导的RNA聚合，并且连接酶构成了一般RNA聚合酶核糖酶的催化核心。 为了了解RNA催化的RNA依赖性RNA聚合的基础，我们已经着手求解连接酶的晶体结构。