MALDI TOF TOF mass spectrometer

MALDI TOF TOF 质谱仪

• 批准号: 6877629
• 负责人: PETER LOBEL
• 金额: $ 50万
• 依托单位: UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877629
• 关键词: biomedical equipment; biomedical equipment purchase; mass spectrometry; matrix assisted laser desorption ionization

DESCRIPTION (provided by applicant): The application of mass spectrometry to the study of biological molecules has revolutionized many aspects of biomedical research. A group of thirty NIH-supported research groups located at UMDNJ-Robert Wood Johnson Medical School and Rutgers, the State University of New Jersey (Piscataway/New Brunswick Campus) are requesting an Applied Biosystems 4700 Proteomics Analyzer MALDI TOF TOF mass spectrometer to replace and upgrade our existing shared MALDI-TOF mass spectrometry resource. The user group members propose to use this instrument to perform biomedically relevant proteomics studies, protein identification, quality control of recombinant proteins, peptides, and bioconjugates, characterization of post-translational and chemical modifications, cross-linking analysis, deuterium exchange, as well as physical and structural biology studies. Most members of the User Group currently employ mass spectrometry as an integral part of their research and require continued access to a MALDI mass spectrometer, and would benefit immensely from the increased capabilities of the requested instrument. This would result in significant increases in productivity. More importantly, this resource will allow the investigators to expand their research in new directions, and to begin new projects that could not have previously been undertaken.

描述（由申请人提供）：质谱在生物分子研究中的应用彻底改变了生物医学研究的许多方面。位于 UMDNJ-Robert Wood Johnson 医学院和新泽西州立大学罗格斯分校（皮斯卡塔韦/新不伦瑞克校区）的由 30 个 NIH 支持的研究小组正在请求使用 Applied Biosystems 4700 蛋白质组分析仪 MALDI TOF TOF 质谱仪来取代和升级我们现有的共享 MALDI-TOF 质谱资源。用户组成员建议使用该仪器进行生物医学相关的蛋白质组学研究、蛋白质鉴定、重组蛋白质、肽和生物缀合物的质量控制、翻译后和化学修饰的表征、交联分析、氘交换以及物理和结构生物学研究。用户组的大多数成员目前将质谱作为其研究的一个组成部分，并需要持续使用 MALDI 质谱仪，并且将从所需仪器的增强功能中受益匪浅。这将导致生产力的显着提高。更重要的是，这一资源将使研究人员能够将他们的研究扩展到新的方向，并开始以前无法开展的新项目。