High Resolution LC-MS/MS System

高分辨率 LC-MS/MS 系统

• 批准号: 7595425
• 负责人: PETER LOBEL
• 金额: $ 50万
• 依托单位: UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595425
• 关键词: Area; Biological; Biomedical Research; Communicable Diseases; Funding; High Pressure Liquid Chromatography; Institution; Liquid Chromatography; Malignant Neoplasms; Mass Spectrum Analysis; Neurodegenerative Disorders; Post-Translational Protein Processing; Proteins; Proteomics; Request for Applications; Research; Research Personnel; Resolution; Solvents; Source; System; United States National Institutes of Health; Universities; Wood material; comparative; crosslink; instrument; instrumentation; mass spectrometer; medical schools; meetings; public health relevance; tandem mass spectrometry

DESCRIPTION (provided by applicant): This is an application requesting funds to purchase instrumentation to conduct high-resolution liquid chromatography tandem mass spectrometry (LC-MS/MS). The instrument will be located in an existing biological mass spectrometry facility on the Piscataway campus of Robert Wood Johnson Medical School (RWJMS) and Rutgers University. A high resolution LC-MS/MS instrument will greatly expand the capabilities of the facility to meet the increasing research needs of 31 NIH-funded investigators. Funds from NIH along with institutions support will allow the acquisition of a ThermoFisher LTQ Orbitrap XL ETD mass spectrometer, a Proxeon nanospray source, and a Dionex Ultimate 3000 HPLC system. There are no comparable instruments at RWJMS and Rutgers. The instrument will be used for characterization of post-translational modifications on proteins, quantitative analysis/comparative proteomics, crosslinking analysis, solvent accessibility studies, and other application that require high-resolution LC-MS/MS capabilities. This will accelerate the pace of basic and applied biomedical research into important areas including neurodegenerative disease, infectious disease, and cancer. PUBLIC HEALTH RELEVANCE: This is an application requesting funds to acquire a specialized mass spectrometer that will be used for projects being conducted by 31 NIH-supported investigators. The availability of this instrument will accelerate the pace of basic and applied biomedical research into important areas including neurodegenerative disease, infectious disease, and cancer.

描述（由申请人提供）：这是一份请求资金购买仪器以进行高分辨率液相色谱串联质谱（LC-MS/MS）的申请。该仪器将安装在罗伯特伍德约翰逊医学院 (RWJMS) 和罗格斯大学皮斯卡塔韦校区现有的生物质谱设施中。高分辨率 LC-MS/MS 仪器将极大地扩展该设施的能力，以满足 31 名 NIH 资助的研究人员日益增长的研究需求。 NIH 的资金和机构支持将允许购买 ThermoFisher LTQ Orbitrap XL ETD 质谱仪、Proxeon 纳米喷雾源和 Dionex Ultimate 3000 HPLC 系统。 RWJMS 和罗格斯大学没有类似的仪器。该仪器将用于蛋白质翻译后修饰的表征、定量分析/比较蛋白质组学、交联分析、溶剂可及性研究以及需要高分辨率 LC-MS/MS 功能的其他应用。这将加快神经退行性疾病、传染病和癌症等重要领域的基础和应用生物医学研究的步伐。公共卫生相关性：这是一份申请，要求提供资金来购买专用质谱仪，该质谱仪将用于 31 名 NIH 支持的研究人员正在进行的项目。该仪器的推出将加快神经退行性疾病、传染病和癌症等重要领域的基础和应用生物医学研究的步伐。