High Throughput Screening

高通量筛选

• 批准号: 7245715
• 负责人: C DAVID WEAVER
• 金额: $ 9.14万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7245715
• 关键词: AXIN2 protein; Adherens Junction; Affect; Arts; Biological; Biological Assay; Biology; Cell Line; Chemicals; Colorectal Cancer; Compatible; Data; E-Cadherin; Goals; Human; Institutes; Medical Research; Physiology; Purpose; Regulation; Reproduction spores; Research Personnel; Role; System; Technology; Work; colon cancer cell line; high throughput screening; in vitro Assay; novel; therapeutic target; tool

High-throughput screening (NTS) is a means of rapidly assessing the ability of hundreds of thousands of chemical compounds to affect biological systems for the purpose of accelerating medical research. We propose an HTS core the goal of which is to enable Gl SPORE investigators to discover chemical tools to promote better understanding of colorectal cancer physiology and potentially to help reveal novel targets and therapeutic opportunities. Using state-of-the-art HTS technologies and working with Gl SPORE investigators, the Vanderbilt Institute of Chemical Biology's HTS facility has developed and validated HTS-compatible assays aimed at discovering chemical tools relevant to: 1) E-cadherin regulation in the human colon cancer cell line, SW620; 2) axin and (3-catenin stability in a novel in vitro assay system; and 3) p120's role in modulating E-cadherin activity and adherens junction formation in the Colo205 cell line. We present data demonstrating our ability to perform the HTS assays, discover compounds that affect the systems noted above, and we detail our plans to use the products of the HTS to further the aims of the Gl SPORE.

高通量筛选（NTS）是一种快速评估数十万的能力的手段 为了加速医学研究而影响生物系统的化合物。我们 提出HTS核心的目标是使GL孢子调查人员发现化学工具 促进更好地了解结直肠癌生理学，并有可能帮助揭示新的目标和 治疗机会。使用最先进的HTS技术并与GL孢子研究人员合作， 范德比尔特化学生物学研究所的HTS设施已开发并验证了HTS兼容 旨在发现与以下方面有关的化学工具：1）人类结肠癌中的E-钙粘蛋白调节 细胞系，SW620； 2）Axin和（新型体外测定系统中的3-Catenin稳定性； 3）P120在P120中的作用 调节Colo205细胞系中的E-钙粘蛋白活性和粘附连接形成。我们提出数据 证明我们执行HTS测定法，发现影响系统的化合物的能力 上面，我们详细介绍了使用HTS产品来进一步进一步的GL孢子目标的计划。