Cincinnati Comprehensive Sickle Cell Center

辛辛那提综合镰状细胞中心

• 批准号: 6530540
• 负责人: Clinton H Joiner
• 金额: $ 180.27万
• 依托单位: CINCINNATI CHILDRENS HOSP MED CTR
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-11 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6530540
• 关键词: clinical research; cooperative study; human subject; sickle cell anemia

The Cincinnati Comprehensive Sickle Cell Center, based in the Division of Hematology/Oncology. at Children's Hospital Medical Center and affiliated with the University of Cincinnati College of Medicine, provides a full range of clinical services to people affected by sickle cell disease. Building on the finding that elevated homocysteine (Hcy) in sickle cell patients is associated with pyridoxine deficiency, an inter-Center collaborative research proposal examines the correlation of elevated Hey with activation of coagulation and endothelial cells in patients, and tests whether pyridoxine supplementation corrects these abnormalities. An interactive research team with expertise in molecular biology, membrane transport, in vivo cell kinetics, and clinical research will pursue projects focused on SS RBC dehydration, a key factor in sickle cell pathology, which results from two abnormal transport pathways, KCl cotransport (KCC) and sickling-induced cation leaks. The clinical study of Project 2 tests the therapeutic potential of dipyridamole, which inhibits sickling-induced cation leaks, and magnesium, which inhibits KCC, to improve SS RBC hydration in vivo. Project 3 examines the rate and mechanisms of hydration changes of SS RBC in vivo, using biotin label techniques unique to this Center. Project 4 explores the molecular basis of post-translational regulation of KCC by cell volume in normal and SS RBC. Project 5 focuses on the erythroid isoforms of KCC and their transcriptional regulation. A Clinical Core includes Pediatric and Adult Progra ms to provide comprehensive services to over 375 patients, to support basic and clinical research efforts, and to participate in inter-Center collaborative trials. An Education and Patient Services Core provides educational and psychosocial support for patients and families, and includes a Transition Program bridging the Pediatric and Adult Clinical Programs, in collaboration with the Sickle Cell Awareness Group and the Urban League of Cincinnati. An Administrative Core provides overall fiscal and programmatic management and serves as the focal point for Center activities, programs, and communication. The projects and cores in this proposal will be integrated with independently funded programs of newborn hemoglobinopathy screening follow-up, education and counseling, a Hemoglobin Diagnostic Laboratory, and other basic science and clinical research projects. The Cincinnati comprehensive Sickle Cell Center is poised to lead in the national effort to develop and test new therapies for sickle cell disease to improve the lives of affected individuals.

辛辛那提综合镰状细胞中心，位于血液学/肿瘤学部门。辛辛那提大学医学院附属儿童医院医疗中心为镰状细胞病患者提供全方位的临床服务。基于镰状细胞病患者同型半胱氨酸 (Hcy) 升高与吡哆醇缺乏相关这一发现，一项中心间合作研究计划检查了 Hey 升高与患者凝血和内皮细胞激活的相关性，并测试补充吡哆醇是否可以纠正这些问题异常。一个在分子生物学、膜运输、体内细胞动力学和临床研究方面拥有专业知识的互动研究团队将开展专注于 SS RBC 脱水的项目，SS RBC 脱水是镰状细胞病理学的一个关键因素，由两种异常运输途径 KCl 共转运 (KCC) 引起。 ）和镰刀引起的阳离子泄漏。项目 2 的临床研究测试了双嘧达莫（抑制镰状细胞诱导的阳离子渗漏）和镁（抑制 KCC）改善 SS RBC 体内水合作用的治疗潜力。项目3利用本中心独有的生物素标记技术，研究SS红细胞体内水合变化的速率和机制。项目 4 探索正常和 SS 红细胞中细胞体积对 KCC 翻译后调节的分子基础。项目 5 重点研究 KCC 的红系亚型及其转录调控。临床核心包括儿科和成人项目，为超过 375 名患者提供全面服务，支持基础和临床研究工作，并参与中心间合作 试验。教育和患者服务核心为患者和家庭提供教育和社会心理支持，包括与镰状细胞意识小组和辛辛那提城市联盟合作的过渡计划，连接儿科和成人临床计划。行政核心提供全面的财务和计划管理，并作为中心活动、计划和沟通的焦点。该提案中的项目和核心将与独立资助的新生儿血红蛋白病筛查随访、教育和咨询、血红蛋白诊断实验室以及其他基础科学和临床研究项目相结合。辛辛那提综合镰状细胞中心准备领导全国开发和测试镰状细胞病新疗法的努力，以改善受影响个体的生活。