MICROBIAL DIVERSITY IN SEDIMENT

沉积物中的微生物多样性

• 批准号: 7357367
• 负责人: MITCHELL L SOGIN
• 金额: $ 0.12万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357367
• 关键词: MICROBIAL; DIVERSITY; SEDIMENT

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. I am studying microbial diversity in sediment samples from various bodies of water (currently Mount Hope Bay). I used the BRC nanodrop to determine the amount of DNA after a PCR reaction targeting the V6 region of 16s RNA. We have a spectrometer across the hall but I tend to get variable results and had heard from others how reliable the nanodrop was. My source of funding is an Astrobiology grant from NAI/NASA to P.I. Mitch Sogin

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是中心的机构，不一定是研究者的机构。我正在研究来自不同水体（目前为希望山湾）的沉积物样本中的微生物多样性。我使用 BRC nanodrop 来测定针对 16s RNA V6 区域的 PCR 反应后的 DNA 量。我们在大厅对面有一个光谱仪，但我往往会得到不同的结果，并且从其他人那里听说纳米水滴有多可靠。我的资金来源是 NAI/NASA 向 P.I. 提供的天体生物学资助。米奇·索金