TRANSGENIC AND CHIMERIC MOUSE CORE

转基因和嵌合小鼠核心

基本信息

批准号：
7284636
负责人：
NANCY E. COOKE
金额：
$ 12.1万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-04-01 至 2012-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7284636
关键词：
Ablation Arts Bacteriophage P1 Biomedical Research Carbohydrates Cell Lineage Cells Clinical Research Collaborations Core Facility Cryopreservation DNA Data Deletion Mutation Development Developmental Biology Diabetes Mellitus Diagnostic Disease Elements Embryo Endocrinology Experimental Models Faculty Financial Contribution Funding Gene Expression Gene Targeting Generations Genes Hereditary Disease Homeostasis Hormones Human Resources Inbred Strain Individual Infection Intracytoplasmic Sperm Injections Islets of Langerhans Knock-out Knockout Mice Laboratories Localized Metabolic Pathway Microinjections Modification Molecular Biology Mouse Strains Mus Mutation Numbers Nutritional Obesity Oocytes Operative Surgical Procedures Pathogenesis Pathway interactions Pennsylvania Personal Satisfaction Phase Physiological Physiology Procedures Purpose Range Regulatory Pathway Resources Secure Services Site Staging Study models System Techniques Technology Transcriptional Regulation Transgenes Transgenic Animals Transgenic Mice Transgenic Organisms Universities base cost day diabetic embryonic stem cell gene function homologous recombination improved in vivo insight interest lipid metabolism medical schools member mouse genome mouse model novel pathogen size sperm cell success tissue culture tool transgene expression tumorigenesis type I and type II diabetes

项目摘要

TRANSGENIC AND CHIMERIC MOUSE CORE: Director-N. Cooke The Transgenic and Chimeric Mouse Core Facility (TCMF) of the University of Pennsylvania has been in operation since February 1992 under the continuous co-direction of Dr. Nancy Cooke and Dr. Stephen Liebhaber and under the day-to-day technical direction of Dr. Jean Richa. The initial components of the TCMF were assembled from financial contributions of the School of Medicine and resources from the laboratories of Drs. Cooke and Liebhaber in 1989 with subsequent phased expansions in facilities, capabilities, personnel, and services. Over the past five years the Core has generated a wide range of transgenic and gene-targeted mouse lines that have proven to be powerful tools for the study of the molecular biology and physiology of Type I and Type II diabetes, obesity, and developmental and mechanistic bases for a wide variety of other disorders of endocrinology and metabolism. The Core laboratory is located in a pathogen-free microbiologic barrier facility in the basement of the Clinical Research Building. A primary function of the TCMF is to provide a centralized laboratory that will generate infectionfree strains of mice carrying transgenes or targeted genetic alterations of specific interest to individual projects in the DERC. The centralization of these technically demanding procedures in the Core laboratory results in enhanced efficiency and significant cost reduction for each project. During the current project period the TCMF has grown significantly in service offerings and its facilities have been improved and expanded. A major increase in the throughput potential of the Core has been achieved by the doubling in the size of the Core microinjection laboratory, funded by the School of Medicine. This expansion has doubled the capacity in line with the continuing increase in demand for services. In addition, the Core is now finalizing a new Cryopreservation Facility for the long-term storage of embryos and sperm. This addition to the Core will be opening in the summer of 2006. This newly configured, secure facility is off-site from the main Core laboratory, and has been established by funds from the School of Medicine. Additional services that have been established during the current funding period include sperm cyropreservation, intracytoplasmic sperm injection (ICSI), DNA diagnostics, and integration with the School of Medicine gene knock-out service. These services have substantially increased the utility of the Core to Center members. The Core will continue to maintain the highest level of quality service and will continue to acquire new techniques and expertise to maintain services at a 'state-of-the-art' level.

转基因嵌合小鼠核心：Director-N。库克宾夕法尼亚大学转基因嵌合小鼠核心设施（TCMF）已在自 1992 年 2 月起在 Nancy Cooke 博士和 Stephen 博士的持续共同指导下运作 Liebhaber 和 Jean Richa 博士的日常技术指导下。的初始组成部分 TCMF 由医学院的财政捐款和来自医学界的资源汇集而成博士的实验室。 Cooke 和 Liebhaber 于 1989 年建立，随后分阶段扩建设施，能力、人员和服务。在过去的五年里，核心已经产生了广泛的转基因和基因靶向小鼠品系已被证明是研究的强大工具 I 型和 II 型糖尿病、肥胖以及发育和发育的分子生物学和生理学各种其他内分泌和代谢疾病的机制基础。核心实验室位于临床研究地下室的无病原体微生物屏障设施内建筑。 TCMF 的主要功能是提供一个集中实验室，以产生无感染的结果携带个体特定兴趣的转基因或靶向遗传改变的小鼠品系 DERC 的项目。这些技术要求较高的程序集中在核心实验室提高每个项目的效率并显着降低成本。在当前项目期间在此期间，TCMF 的服务范围显着增长，其设施得到改善，扩大了。通过双倍处理，核心的吞吐量潜力显着增加由医学院资助的核心显微注射实验室的规模。这个扩展有随着服务需求的持续增长，容量增加了一倍。此外，现在的核心是最终确定用于长期储存胚胎和精子的新冷冻保存设施。此添加至核心区将于 2006 年夏季开放。这个新配置的安全设施位于主要核心实验室，由医学院资助建立。附加服务在当前资助期间建立的项目包括精子冷冻保存、胞浆内单精子注射 (ICSI)、DNA 诊断以及与医学院基因整合淘汰赛服务。这些服务大大提高了核心成员对中心成员的效用。 The Core将继续保持最高水平的优质服务，并将继续获取新的将服务维持在“最先进”水平的技术和专业知识。